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repaq's Issues

俩示例文件是啥?

repaq使用的俩示例文件是啥?DNA/RNA?人/其他物种?NA12878?想拿这个数据尝试一些好玩的东西,希望了解这个数据的更多信息

Error Info: Segmentation fault (core dumped)

Hello,

Recently, I used repaq to compress a fastq file and then tried to check the result. When I used this cmd: "repaq -p -i xxxxxxx.fq.gz -r test.rfq -j test.txt", it returned "Segmentation fault (core dumped)" and stopped. Could anyone help me to fix it? Thx~

failed to call xz, despite it definitely being on the system

I get the following error message immediately:

xz: (stdin): Cannot allocate memory
ERROR: failed to call xz, please confirm that xz is installed in your system

when I run:

sbatch -c 16 --mem 10000 --wrap "repaq -c -i file_1_trimmed.fastq.gz -I file_2_trimmed.fastq.gz -o file.fpq.xz --compression 9 --thread 16"

I installed repaq and xz with the following commands in a fresh conda environment:

conda install -c bioconda repaq
conda install conda-forge::xz

Help would be much appreciated

Consistency of repaq, and readiness for production

Hi! We're considering using repaq due to the significant space savings, but have some concerns about lossless-ness. Some of the other issues (#11, #12, #13) seem to suggest that repaq isn't lossless, but there isn't enough detail on some (#12, #13) to determine the severity.

How production ready is repaq?

MD5 FAIL

Hello, I recently found some problems using repaq-0.3.0.
After decompressing, I found that the md5 check failed. Comparing the decompressed fastq file with the original file, it was found that at some point in some reads N became G after being compressed. Is this a machine problem or an algorithm problem? I compressed 160 files and 20 of them were problematic. Recompressing these 20 is still the same result.
Here is the result of the --compare parameter
"result":"failed", "msg":"The RFQ file and FASTQ file have different sequence in the 7815 pair. GGACCTCTTCTGACTGATGGGAAATCACAGCAGTTGGAGACCCAGGTCCACAGGAAGGATGAAGAACCCAAGGAATGGCAGCAGACTGAGAGCTTCTGGA | GGACCTCTTCTGACTGATGGGAAATCACAGCAGTTGGAGACCCAGGTCCACAGGAAGGATGAAGAACCCAAGNAATGGCAGCAGACTGAGAGCTTCTGGA"

round-trip is not identical

On some fq files, the quality string is not preserved
in a round-trip conversion. In particular ; or : gets replaced by I.

One example line, first line is original:

-BBDADFHHHHHHII?HHHIIII?EHEHCDHDHGFHHHEFHHHIECD/E?@FH1<@GEGH?GHH?@CC@?E1<D@FGH111G?CHF0CCEG?0<FFE0:F0=
+BBDADFHHHHHHII?HHHIIII?EHEHCDHDHGFHHHEFHHHIECD/E?@FH1<@GEGH?GHH?@CC@?E1<D@FGH111G?CHF0CCEG?0<FFE0IF0=

repaq-command

repaq -c -i original.fq -o original.fq.rfq

is it possible make bcl2rfq in progress

since new version of bcl2fastq exist many problems in practical applications, besides, fastp is an amazing utility and it has came into use in my pipelines.

I guess it is a great work that we can develop an alternative tool in the very beginning of the analysis workflow.

Once it works, maybe a new workflow of raw data processing standard is born.

decompression error

Hi, thanks for the tool! The compression ratio is IMPRESSIVE, down to 3.1%! But I got the following error using decompression...
"
~/bin/repaq -d -i haha.rfq -o ha_1.fq -O ha_2.fq
terminate called after throwing an instance of 'std::out_of_range'
what(): basic_string::substr: __pos (which is 404978952) > this->size() (which is 404978876)
Aborted (core dumped)
"

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