Comments (2)
hi @hansong798, thank you so much for your interest in SPOTlight and for reaching out!
Would it be possible to see how the topic profiles look for each cell type? Maybe there is an overlap between the topics learned for CAFs and tumor cells and the former is capturing all the signal. It would also be interesting to look at which genes are the most important for the topics associated with tumor cells and assess if they makes sense.
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Dear Marc,
Thank you for developing SPOTlight! Really love the idea of NMF and the visualization of results!
I faced the same issue while training the model on the whole atlas. When I increase the number of cells included in reference object, the predicted cell type proportion changed drastically. May I know your thoughts on this? In your paper, I noticed you separately trained the model on a more specific immune atlas. Do you think that will help in this case? Also, how do you usually filter the markers you feed the model? Do you filter for positive logFoldChange genes and limit to maybe top 50 or 100 genes? for weightage of gene, do you recommend using pvalues or logfoldchange (do we include negative values?)?
Sorry for so many questions! Would really appreciate to know your thoughts/recommendations on these
Thank you again!
Best Regards,
Justine
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Related Issues (20)
- export SPOTlight results to 10x Genomics Loupe Browser HOT 3
- colData(sc_data)$subclass HOT 1
- Difference between cell type correlation and cell communication HOT 1
- SPOTlight with low numbers of genes per spot HOT 1
- Does the new version of SPOTlight no longer support data from Seurat? HOT 1
- ERROR: dependency ‘arrangements’ is not available for package ‘SPOTlight’ HOT 4
- test_spot_fun HOT 2
- Error in trainNMF(x = x, y = y, groups = groups, mgs = mgs, n_top = n_top, : ids %in% names(mgs) are not TRUE
- Parallel run HOT 2
- Error slot %in% SummarizedExperiment::assayNames(x) is not TRUE HOT 3
- function plotSpatialScatterpie with image=TRUE does not work HOT 4
- plotInteractions HOT 8
- What does the 'decon_df' mean? HOT 5
- Error in .extract_counts(y, assay, slot) : !is.null(colnames(x)) is not TRUE HOT 2
- ST technology (the predecessor of 10X Visium technology) HOT 1
- Error in `SeuratObject::GetAssayData()`: HOT 4
- plotSpatialScatterpie HOT 9
- assay %in% SeuratObject::Assays(x) is not TRUE HOT 1
- Q - Visium analysis - subset image and spot demultiplexing HOT 5
- packages version HOT 3
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