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License: MIT License
Dear zheng:
When we prepared the RNASeq_pipeline, we encounted an error with running this CMD $gffread NONCODEv5_human_hg38_lncRNA.gtf -g Homo_sapiens_assembly38.fasta -w NONCODEv5_human_hg38_lncRNA.fa
from "prepare_reference/run_reference.sh line 41". We have replaced the Homo_sapiens_assembly38.fasta
to GRCh38.primary_assembly.genome.fa
, but it didn't work and output an error Warning: couldn't find fasta record for 'chr4_GL000008v2_random'! Error: no genomic sequence available (check -g option!).
. Would you like to help us to work out how to create this file Homo_sapiens_assembly38.fasta
?
Thank you so much!
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