I'm trying to use flopR to analyze a few .fcs files for the automated features it provides. I have installed the package using devtools::install_github()
and ensured the prerequisites c("flowCore", "flowClust", "flowStats")
are installed.
[1] "2 clusters found"
[1] "only debris found"
Error 'x' must be an object of class flowFrame
and some additional warning messages reg NaNs and fluorescent channel exceeding saturation
How do I go about troubleshooting this since no additional information is given about the function generating the error?
I am able to read and visualize the same files using flowWorkspace::'s cytoset and cytoframe datatypes like this -