The following document describes how to utilize the Growth Rate workflow.

The following details information on how to use and fill out the Plate Map Excel file.

1. Sheet “PlateMap”

• This sheet is reserved for your 96-well plate map design; in the 60-cells (clear coloured cells), enter your treatment type for each specific well. I.e Well 1 = cell B2 = LKB1 KnockDown + 75nM Radicicol.

2. Sheet “Drugs”

• This sheet is reserved for any Drug treatments used in your experiment. Enter the drug name under column header ‘drug’ and enter the corresponding type, being a ‘Control’ or ‘Treatment’.

3. Sheet “Timepoints”

• This sheet is used to keep track of the dates and times of DPC measurements; to calculate each time point.

4. Sheet “Conc.”

• This sheet is used calculate and prepare drug concentrations.

5. Sheet ‘To Print”

• This sheet is used to print experimental plate map and drug concentration table

The following details information on how to use and fill out the Dataset Excel file.

1. Sheet “Data”

• Field Header “Path to Dataset” ** Enter the full path to the location of the folder containing your ResultsTable Data. ** i.e. \carbon.research.sickkids.ca\rkafri\OPRETTA\Operetta Processed OutPutFiles\Dataset_20171204_JS_cycE_6HrRESULTS
• Field Header “Plate_Map” ** Enter the full path to the location of the folder containing your Plate Map Excel File. ** i.e. \carbon.research.sickkids.ca\rkafri\Justin S\Experiments\Growth Rate\Plate map 20171204_cycE1.xlsx
• Field Header “Sheet”: ** Enter the sheet name that contains your experimental platemap. i.e. PlateMap
• Field Header “Range” ** Enter the cell range that contains your 60-well experimental information. i.e. B2:K7
• Field Header “Sheet2” ** Enter the sheet name that contains all the names of the drugs used in your experiment. i.e. Drugs
• Field Header “Exp_Name” ** Enter a unique experiment name for each unique experiment. i.e. 20171204_JS_cycE.
• Field Header “Time-Point” ** Enter the corresponding time point to the corresponding ResultsTable dataset.
• Field Header “Cell Line” ** Enter the cell line for each corresponding dataset. i.e. RPE1
• Field Header “Expression” ** If your cell lines are specific inducible cell lines, enter the specific type of inducible expression. i.e. cycE. ** You can enter more than one, but they must be separated by a comma (,). i.e. cycE,cycD,p27 ** In your case, you could put LKB1neg or LKB1pos.
• Field Header “Imaging_Type” ** Enter the type of imaging measurement, either DPC or SE.
• Field Header “Date” ** Enter the date of experiment.

2. Sheet “MasterSheet”

• Don’t worry about this sheet.