Comments (6)
You need to install the latest RProtoBufLib
and cytolib
from cytoml.
Thank you, this did the trick:
install_github("RGLab/RProtoBufLib", ref="trunk")
install_github("RGLab/cytolib", ref="trunk")
install_github("RGLab/flowWorkspace", ref="trunk")
from cytoml.
Actually, the flowWorkspace package still gives me an error:
clang++ -std=gnu++11 -dynamiclib -Wl,-headerpad_max_install_names -undefined dynamic_lookup -single_module -multiply_defined suppress -L/Library/Frameworks/R.framework/Resources/lib -L/usr/local/lib -o flowWorkspace.so R_API.o R_GatingHierarchy.o R_GatingSet.o RcppExports.o getSingleCellExpression.o setCounts.o /Library/Frameworks/R.framework/Versions/3.4/Resources/library/RProtoBufLib/lib/GatingSet.pb.o /Library/Frameworks/R.framework/Versions/3.4/Resources/library/RProtoBufLib/lib/libprotobuf.a -lxml2 -lz -lpthread -licucore -lm -F/Library/Frameworks/R.framework/.. -framework R -Wl,-framework -Wl,CoreFoundation
ld: warning: ignoring file /Library/Frameworks/R.framework/Versions/3.4/Resources/library/RProtoBufLib/lib/GatingSet.pb.o, file was built for unsupported file format ( 0x7F 0x45 0x4C 0x46 0x02 0x01 0x01 0x00 0x00 0x00 0x00 0x00 0x00 0x00 0x00 0x00 ) which is not the architecture being linked (x86_64): /Library/Frameworks/R.framework/Versions/3.4/Resources/library/RProtoBufLib/lib/GatingSet.pb.o
if [ uname -s
= 'Darwin' ]; then install_name_tool -change /usr/local/clang4/lib/libc++.1.dylib /usr/lib/libc++.1.dylib flowWorkspace.so; fi
installing to /Library/Frameworks/R.framework/Versions/3.4/Resources/library/flowWorkspace/libs
** R
** inst
** tests
** preparing package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded
Error: package or namespace load failed for ‘flowWorkspace’ in dyn.load(file, DLLpath = DLLpath, ...):
unable to load shared object '/Library/Frameworks/R.framework/Versions/3.4/Resources/library/flowWorkspace/libs/flowWorkspace.so':
dlopen(/Library/Frameworks/R.framework/Versions/3.4/Resources/library/flowWorkspace/libs/flowWorkspace.so, 6): Symbol not found: __ZN2pb11ellipseGate17default_instance_E
Referenced from: /Library/Frameworks/R.framework/Versions/3.4/Resources/library/flowWorkspace/libs/flowWorkspace.so
Expected in: flat namespace
in /Library/Frameworks/R.framework/Versions/3.4/Resources/library/flowWorkspace/libs/flowWorkspace.so
Error: loading failed
Execution halted
from cytoml.
Sorry, included the object file in repo by mistake. Try to re-install RProtoBufLib
from github.
from cytoml.
Ok, I was able to install the libraries from github. I now have:
flowWorkspace version 3.27.6
openCyto version 1.17.2
flowCore version 1.45.2
My original problem was not being able to import a Gating-ML 2.0 file from Cytobank into R, the error: Error in FUN(X[[i]], ...) :
Don't know how to inverse transformation: logtGml2
I was hoping the updated libraries would be able to deal with that, but the error persists.
from cytoml.
see #30
from cytoml.
Related Issues (20)
- CytoML Xml File Parsing Issues HOT 8
- Issue Parsing FlowJo (v10.7.2) XML File HOT 3
- FCS Express compatibility HOT 7
- Get indices of gated events from a FJ workspace / alternative to flowjo_to_gatingset HOT 5
- gs-to-flowjo Singularity issue HOT 2
- Ellipses coordinates are not correct in Gating Set after flowJo workspace parsing - v2.9.0 HOT 4
- R Session Aborts when calling flowjo_to_gatingset() more than once HOT 3
- gatingset_to_flowjo() fails with unsupported transformation: LIN
- Using FlowJo gating on pre-compensated, pre-transformed FCS files HOT 5
- flowjo_to_gatingset troubleshoot: "unknown tranformation type!transforms:linear" HOT 3
- flowjo_to_gatingset(), colname not found: error HOT 2
- Export populations as dataframe from a FlowJo workspace HOT 2
- extract inverse transform
- flowjo_to_gatingset does not accept data.frame as path HOT 5
- flowCore::read.FCS works but CytoML::flowjo_to_gatingset fails; no valid FCS file HOT 11
- boolean gate error
- Incompatible with Apple Silicon Mac HOT 7
- flowjo_to_gatingset ignore range as defined in FCS files HOT 3
- Support for FCS 3.2? HOT 9
- gatingset_to_flowjo hangs HOT 1
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from cytoml.