Comments (5)
right, the feature of passing path as a data.frame was deprecated as we re-factored entire parsing code into c++, because it wasn't widely used functionality and we didn't think it was worthwhile to port it.
I have updated documentation to reflect the current state of this parameter.
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Wonderful, thanks for the info and the update.
I have a situation where I need to explicitly define the fcs files, as there are duplicates of the same filename in two folder in the folder structure from the wsp.
Without this functionality, what is the best way to open the wsp? Can I iterate over the fcs files one by one, or would you suggest something else?
from cytoml.
For my purposes, I do iterate over fcs file paths and import them separately as gating sets with CytoML::flowjo_to_gatingset.
When your files (or now gatingsets) are consistent and you want to continue with the gatingset format, you may use flowWorkspace::merge_list_to_gs() to merge a list gatingsets into one gatingset object.
Is it only the filenames that are duplicated or also the meta data of respective FCS files? If it is only the filenames but meta data are unique to each file then you may use the "subset" argument of CytoML::flowjo_to_gatingset to explicitly direct the function to the desired fcs file. The "path" argument may then be the root folder of your fcs files.
There may be a few more details to consider and it may still be a bit tricky though ...
from cytoml.
Thanks for the input, much appreciated. I'll run with your suggestions and figure it out.
As such, no longer an issue. Closing.
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type ?flowjo_to_gatingset and look for section on additional.keys
parameter that can be used to address fcs file matching problem
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Related Issues (20)
- CytoML Xml File Parsing Issues HOT 8
- Issue Parsing FlowJo (v10.7.2) XML File HOT 3
- FCS Express compatibility HOT 7
- Get indices of gated events from a FJ workspace / alternative to flowjo_to_gatingset HOT 5
- gs-to-flowjo Singularity issue HOT 2
- Ellipses coordinates are not correct in Gating Set after flowJo workspace parsing - v2.9.0 HOT 4
- R Session Aborts when calling flowjo_to_gatingset() more than once HOT 3
- gatingset_to_flowjo() fails with unsupported transformation: LIN
- Using FlowJo gating on pre-compensated, pre-transformed FCS files HOT 5
- flowjo_to_gatingset troubleshoot: "unknown tranformation type!transforms:linear" HOT 3
- flowjo_to_gatingset(), colname not found: error HOT 2
- Export populations as dataframe from a FlowJo workspace HOT 2
- extract inverse transform
- flowCore::read.FCS works but CytoML::flowjo_to_gatingset fails; no valid FCS file HOT 11
- boolean gate error
- Incompatible with Apple Silicon Mac HOT 7
- flowjo_to_gatingset ignore range as defined in FCS files HOT 3
- Support for FCS 3.2? HOT 4
- gatingset_to_flowjo hangs
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