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nf-core/phyloplace is a bioinformatics best-practice analysis pipeline that performs phylogenetic placement with EPA-NG.

Home Page: https://nf-co.re/phyloplace

License: MIT License

HTML 3.29% Nextflow 35.96% Groovy 60.75%
nextflow nf-core pipeline workflow phylogenetics phylogenetic-placement evolution sequence-classification taxonomy-assignment evolutionary-tree

phyloplace's People

Contributors

erikrikarddaniel avatar jfy133 avatar

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phyloplace's Issues

MultiQC

Description of feature

Not called at the moment, what can be done?

General classification

Description of feature

Given a single query fasta file with sequences and a set of hmm files with corresponding reference tree and alignment, search the query sequences with all hmm files. Among hits that qualify above a bitscore and/or e-value cutoff, identify the hmm(s) for which each sequence scored best (bitscore). For every hmm, pass the assigned sequences to placement.

The pipeline will be called with a samplesheet without the queryseqfile. Query sequences will instead be passed via --queryseqfile. This combination of parameters, i.e. --input in combination with --queryseqfile, will trigger the new behaviour. Parameters, passed to hmmsearch, for minimum bitscore and maximum e-value will be added.

Start with hmm file

Description of feature

Add the possibility to specify an hmm file that both the reference and query sequences are aligned to.

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