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LuyiTian avatar LuyiTian commented on June 19, 2024

for the 10x, I think you might refer to the wrong reference. We had two batch of 10x data. The first batch have around 900 cells (3 cell lines), second have around 4000 cells(5 cell lines). For sc_CEL-seq2 5 cell line mixtures (3X384 well plate), there are more doublets than we would expect so we exclude this data when we compare some methods, such as clustering. The Drop-seq data contains around 200 cells.
The gene count matrixs were acquired by scPipe, which contains top X cells ranked by reads and it does not perform QC in preprocessing step. So the cell number in gene count matrix does not reflact the real cell number, just result of the parameter.

from sc_mixology.

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