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License: GNU General Public License v3.0
In silico taxonomic classification of Bacillus cereus group genomes using whole-genome sequencing data
License: GNU General Public License v3.0
Hi @lmc297,
I get the following error when trying to install BTyper3
:
Collecting package metadata (current_repodata.json): done
Solving environment: failed with initial frozen solve. Retrying with flexible solve.
Solving environment: failed with repodata from current_repodata.json, will retry with next repodata source.
Collecting package metadata (repodata.json): done
Solving environment: failed with initial frozen solve. Retrying with flexible solve.
Solving environment: /
Found conflicts! Looking for incompatible packages.
This can take several minutes. Press CTRL-C to abort.
failed
UnsatisfiableError:
I'm using a fresh installation of anaconda on windows 11.
Hello
can you provide a tagged release please.
regards
Eric
Hi Laura,
I was installing this great tool this morning, but came across an error with libgsl.so.25.
$ btyper3 -i 2218710072.fna -o bytper3
Welcome to BTyper3!
You are initializing this run at 2022-07-19 08:56
You ran the following command:
/phe/tools/miniconda3/envs/btyper3/bin/btyper3 -i 2218710072.fna -o bytper3
Report bugs/concerns to Laura M. Carroll, [email protected]
Using FastANI to assign 2218710072 to a species at 2022-07-19 08:56
fastANI: error while loading shared libraries: libgsl.so.25: cannot open shared object file: No such file or directory
Traceback (most recent call last):
File "/phe/tools/miniconda3/envs/btyper3/bin/btyper3", line 10, in <module>
sys.exit(main())
File "/phe/tools/miniconda3/envs/btyper3/lib/python3.10/site-packages/btyper3/__init__.py", line 441, in main
run_pipeline(args)
File "/phe/tools/miniconda3/envs/btyper3/lib/python3.10/site-packages/btyper3/__init__.py", line 105, in run_pipeline
final_species = get_species.run_fastani("species", fastani_path, infile, final_results_directory, prefix)
File "/phe/tools/miniconda3/envs/btyper3/lib/python3.10/site-packages/btyper3/ani.py", line 60, in run_fastani
proc.check_returncode()
File "/phe/tools/miniconda3/envs/btyper3/lib/python3.10/subprocess.py", line 456, in check_returncode
raise CalledProcessError(self.returncode, self.args, self.stdout,
subprocess.CalledProcessError: Command '['fastANI', '-q', '2218710072.fna', '--rl', '/tmp/tmp69n8g9vktxt', '-o', 'bytper3/btyper3_final_results/species/2218710072_species_fastani.txt']' returned non-zero exit status 127
After a quick google, the current gsl version 2.7.1 only provides libgsl.so.27. For anyone else that encounters this issue, I fixed it by downgrading the gsl version from 2.7.1 to 2.7.
conda install gsl=2.7
Have a good day!
Caitlin
To enable further follow-up work on the exact files used for comparison, for example for the genomes using ANI, could a feature be added to specify an outpath where the files are stored?
$ python download_pubmlst_latest.py
Downloading most recent PubMLST datbase at 2021-04-12 14:38
Traceback (most recent call last):
File "download_pubmlst_latest.py", line 74, in <module>
main()
File "download_pubmlst_latest.py", line 67, in main
download_pubmlst(btyper3_path)
File "download_pubmlst_latest.py", line 28, in download_pubmlst
tree=ET.parse(xml)
File "/home/domeni/anaconda3/lib/python3.7/xml/etree/ElementTree.py", line 1197, in parse
tree.parse(source, parser)
File "/home/domeni/anaconda3/lib/python3.7/xml/etree/ElementTree.py", line 598, in parse
self._root = parser._parse_whole(source)
xml.etree.ElementTree.ParseError: no element found: line 1, column 0
I'm not very familiar with the requests
and the xml
modules this error seems to be related with. Any clue?
Thank you
Domenico
ANI seems not to be working for me. Using the tutorial data, I got the attached results, with no ANI classification. I also attach the log.
I tried a few genomes but never got an ANI classification, even with the "--ani_typestrains" True parameter
JHQN01.1.log
JHQN01.1_final_results.txt
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