The Cellenics data analysis tasks executor. It consists of two containers: a Python container and an R container. For more details on the individual containers, check out the README files in their respective directories.

The Python part of the worker is a wrapper around the R part: it receives tasks from the API, parses them, sends them to the R part for computation, then formats the results, uploads them to S3 and sends a notification to the API via Redis-backed socket connection that they are ready.

The R part of the worker computes single cell analysis tasks on a pre-processed Seurat rds object, loaded into memory from S3. The R part of the worker can communicate only with the Python part of the worker.

More specific details about the Python or the R part of the worker can be found in the README file in the respective folder (python/ or r/).

The worker is deployed as a Helm chart to an AWS-managed Kubernetes cluster and runs on a Fargate-managed node. The Helm chart template for the worker is located in chart-infra/ folder.

The deployment of the worker is handled by the cluster Helm operator and the worker Github Actions workflow.

During a deployment, the worker Github Actions workflow does the following:

• It pushes new worker images to ECR.
• Adds deployment-specific configurations to the worker Helm chart. Pushes those deployment-specific configuration changes in releases/ folder in iac, under the relevant environment.

Make sure that sufficient resources are allocated in Docker to be able to compile everything. 10gb RAM and 20gb disk image size should be more than enough. Be mindful when allocating RAM, too much and you could end up freezing your computer.

Create a Github Access Token and export it as an environment variable before building. You should consider adding it to your .bashrc (or .zshrc) for convenience.

export GITHUB_API_TOKEN=<your-token>

Assuming the containers are running, you can execute the (pytest) unit tests using:

make test

See here for more information about the tests.

To shut down the development containers, you can use:

make kill

Development is done inside a development container that is automatically built, run, and managed by Visual Studio Code. You do not need R, R Studio, or a Python virtual environment to be installed locally.

As such, you must have the Remote - Containers extension installed. Make sure you restart VS Code after installing to make sure it loads successfully. You should see a green icon in the leftmost part of the status bar, which indicates that the remote container plugin has been installed.

To open the R workspace, you can type code r/r.workspace while in the terminal inside VS Code.

Similarly, to open the Python workspace, you can type code python/python.code-workspace.

You should be prompted to run the workspace inside a container. Accept this. Once you see the folder structure, the worker is running and you have access to the R worker's container. If you get an error after trying to run the workspace inside a container, try running make build to see where exactly the build breaks. Please check Troubleshooting section that lists commonly occuring problems.

The root directories of each of the workspaces are dynamically linked to /r and /python respectively. The terminals spawn terminals within the containers, as expected.

These development environments should be pre-configured with the same requirements as the produciton instances, as well as the necessary VS Code extensions required to debug and lint code.

To run the worker locally, you will need to build it and then run it, passing the id of the processed experiment that you want to use the worker with.

While in the worker/ root folder on the host, you can use make build.

To build and run the R and python containers, you can do:

make build

Note that during the first time, the build can take up to 40-50 minutes to complete. If you get an error, see the Troubleshoooting section for help.

To get a development log stream of both containers running, you can use:

make logs

Before running the worker, you need to have a folder named with the experiment id that you want to load. The folder should be saved under worker/data and it has to contain:

• Processed rds object file, called r.rds
• A json file of the cell_sets for that experiment, called cell_sets.json

Here is an example folder structure for experiment id 1234:

data
├── 1234
│   ├── cell_sets.json
│   └── r.rds

You can obtain this folder structure if you do either of the following:

• Start the rest of the Cellenics components locally, then upload samples from Data Management and launch analysis. A prerequisite for this step is configuring the pipeline to run locally.

OR

• Download the r.rds object and the cell_sets.json file for an already processed experiment from S3 and then manually add them in worker/data under a new folder named with the experiment id.

You can have one or more experiments under worker/data.

To run the worker with the experiment id of your choice, do the following: In a terminal, while in the worker/ root folder, type the following:

EXPERIMENT_ID=1234 make run

where 1234 is the experiment id of your choice.

1. Errors saying ... unsupported option: 'target' after running make build.

   This is most likely a problem with the docker-compose version (which is used by make to build the images). Simply re-install it:

    pip3 uninstall docker-compose
    pip3 install -U docker-compose
   

2. make build fails due to rate limit errors.

   To fix this one, make sure you create a personal access token in your Github account and add it as an environment variable, called GITHUB_PAT:

   1. Go to https://github.com/settings/tokens, create a new token. The token should be read only.
   2. Set `GITHUB_PAT` to equal to the value of the token in a terminal.
   

3. Error: Failed to install 'unknown package' from Github: Timeout was reached: [api.github.com] Resolving timed out after 10000 milliseconds

   This error is due to a bug in DNS resolution of Alpine-based containers running on early releases of Docker Desktop for Mac version 3.

   To fix this, you can download and use a previous version of Docker (e.g. 2.5.0.1) from https://docs.docker.com/docker-for-mac/release-notes/

4. Error when attempting to start the worker saying something like: botocore.exceptions.EndpointConnectionError: Could not connect to the endpoint URL: "http://host.docker.internal:4566/biomage-source-development?...

First, check inframock is running. If it isn't, start it and try again. Otherwise, see below.

Note this error should already been handled by the Makefile builds. If you encounter it while using make run, report it in the Slack channel #engineering.

Go to docker-compose.yaml In the python and r entries add at the end:

extra_hosts:
      - "host.docker.internal:host-gateway"

IMPORTANT: Don't include this in a PR, because it will break stuff on macOS.

TLDR: Save anything in /debug in the container and it will be available at $(pwd)/data/debug.

To save the req argument to a worker function, specify DEBUG_STEP. DEBUG_STEP can be either all (will save req from any task) or the basename of a path in work.R and will hot-reload if changed in work.R. It can also be set on initial run:

# e.g. DEBUG_STEP=getClusters
DEBUG_STEP=task_name make build && make run

When a worker function is run, it will save the req and data objects used by the specified task_name in ./data/debug. You will see a prompt to read these into your R environment:

# clicking these files in RStudio does this for you
req <- readRDS('./data/debug/{experiment_id}_{task_name}_req.rds')
data <- readRDS('./data/debug/{experiment_id}_data.rds')