Comments (5)
Hello hello,
Ahh this is a Windows-specific thing, as I only really use stitchr on Linux and OSX. However I think I know what the problem is (Windows using back- instead of forward-slashes for paths).
Before I try to fix that, does stitchr -dd
work for you? If so could you let me know what path it gives.
from stitchr.
Thank you for the prompt response. Unfortunately, stitchr -dd
doesn't return anything.
I was also wondering if it would be possible to import stitchr
and use it in a Python script or Jupyter Notebook. Thanks again!
from stitchr.
Fixed in e2d7895, v1.1.2, tested on Ubuntu/Mac OSX/Windows.
This also fixes importing stitchr to use in other scripts, which got broken in the conversion to a package. I wrote a quick synopsis of how to start doing so at https://jamieheather.github.io/stitchr/importing.html
The changes have been pushed to PyPI, so you should be able to upgrade or re-download with pip to get the changes.
from stitchr.
Awesome, thank you @JamieHeather!
I was wondering if it is possible to use stitchr
for BCRs?
from stitchr.
You're very welcome - it was something I needed to get to sooner or later anyway!
So in theory yes it absolutely can be used for BCRs, which we demonstrate in the paper (and which has a section in the docs. However as everything is much more complicated in BCRs (and me not being a BCR expert) this isn't something that I'm able to fully support as I can with TCRs.
Generally speaking, what with greater levels of germline polymorphism and SHM I'd recommend using longer reads with the seamless option, and/or ensuring coverage of the relevant genes via the additional genes file. Either way when working with BCRs you might end up having to get under the hood a bit more. Happy to chat about specific use cases if the guidance in the docs isn't enough.
from stitchr.
Related Issues (20)
- Simplifying output / silent mode HOT 1
- J/C region broken in certain genes in skip/extra gene mode HOT 1
- Lower case CDR3 amino acid characters cause an error HOT 1
- TRDV2 error HOT 2
- Possible to use custom species (non-human/non-mouse)? HOT 1
- Alternative non-templated codon usage?
- Improve % of perfectly replicated CDR3s when using a NT CDR3
- Add -cite option to stitchr
- thimble function gives an error for LEADER sequence when stitching TCR HOT 3
- CDR1/2 HOT 2
- Wild card usage in Thimble ignores extra genes (-xg/additional-genes.fasta)
- First example at https://jamieheather.github.io/stitchr/installation.html not working as expected HOT 2
- Importing stitchr for use in other scripts - obtain stitched aa sequence without C region information HOT 5
- Specify databases location HOT 5
- Add option to read FASTA automatically into additional-genes.fasta
- Add more details to docs about different error/warning messages HOT 1
- TRBD gene HOT 2
- Integration with tidytcells HOT 4
- A issue about stitching immunoglobulins HOT 1
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