This project provides user-friendly interactive tools to perform enrichment analysis for multiple datasets across multiple public databases. The instructions below describe how to run these tools and all of their features.

tldr; Just perform the bold, bullet list items below to get up and running fast.

You only have to do this once. Skip this if you've already done this recently.

• Install the latest versions of R and RStudio. Follow the steps provided on that page and then return here.

Note: At a minimum you will need the version of R required by Bioconductor.

The launch_app.R script will check your R dependencies and launch an interactive session.

• Open RStudio
• Copy/paste these lines into the Console and hit return:

if(!require(devtools)) install.packages("devtools"); library(devtools)
options(shiny.launch.browser = .rs.invokeShinyWindowExternal)
source_url("https://raw.github.com/gladstone-institutes/Interactive-Enrichment-Analysis/main/launch_app.R")

This will install some basic dependencies, download the project to your Working Directory (Files tab in RStudio) and launch the tool in your browser.

Advanced: Alternatively, you can git clone this repo and periodically perform git pull to update the code and database collections. Install the minimal dependencies listed in launch_app.R and launch from RStudio (i.e., open any of the shiny_run/app/ files and choose Run App > Run in Window

A collection of public databases is provided and updated monthly as a datestamped (YYYYMMDD) RData file, e.g., 20210108.RData. Cite and provide this file for reproducible research. Download updated versions (or perform git pull) to access the latest content from public databases.

• In the Databases panel, choose a database collection.

The app will take a moment to load the databases, check their validity, and list them for your review. This step is complete when you see ✔ Ready!

Advanced: If you want to construct your own database collection, then add one or more GMT files with NCBI gene identifiers to shiny_run/databases/gmts/ and choose BUILD NEW DATABASE in the Databases panel.

Pro-tip: Between projects, be sure to update your RData or GMT files as new versions are released.

A sample dataset E-GEOD-30573.csv (Voineagu 2011) is provided to illustrate the required format. A gene column is always required. Columns named rank, p.value, and fold.change (log2FoldChange) are also recognized for additional analysis options. All other columns are ignored.

• In the Datasets panel, choose one more more datasets to analyze or choose ADD NEW DATASETS to upload your own CSV files.

You can choose as many dataset files as you like as long as they are all of the same format. Datasets with different formats should be analyzed in separate runs.

The supported formats include:

 - a simple list of genes (for ORA only)
 - a list of genes with a `rank` column (for GSEA only)
 - a list of genes with a `p.value` column (for GSEA and ORA)
 - a list of genes with `p.value` and `fold.change` columns (for GSEA
and ORA)

Caution: For a given run, all datasets are assumed to have the same format. If you have different types of dataset files (per the list above), then you should run them in separate batches.

The app will display a preview of the first few rows of the first chosen dataset along with the required and optional columns it could detect (see table and subsequent message in screenshot).

Identifiers

Supported gene identifiers include SYMBOL, ENSEMBL, ENTREZID, and UNIPROT, by default. But it is trivial to add support for any orgDb keytypes. Simply edit the supported.idTypes list in the "Defaults and Parameters" section of shiny_run/app/global.R.

Organisms

Supported species include human and mouse, by default. In order to add support for any orgDb species, edit the supported.org list in the "Defaults and Parameters" section of shiny_run/app/global.R and generate a collection of databases from GMTs with NCBI Gene identifiers.

Gene set min/max

These options are defined by clusterProfiler and are used to prune the databases, restricting the gene sets considered in the analysis based on their size, e.g., excluding gene sets with fewer than 3 genes or more than 500 genes.

ORA Cutoffs

If ORA is to be performed, then fold.change and p.value cutoffs must be supplied. These are used to define the subset of significantly changed genes to test against all other genes in the dataset.

At the bottom of the panel, the "Analysis plan" will state which analyses will be performed. The app performs all possible analyses. This step is complete when you see ✔ Ready!

When Databases and Datasets are loaded and verified, a third step will appear in the side panel.

If the analysis plan looks good to you, including all the parameter choices, then...

• Click Let's go!

The initial set of panels will collapse and a new Progress panel will open along with a progress bar in the lower-right corner.

It will also report the start and finish times for the run, and the location of the output folder that is given a unique timestamp-based name to support multiple independent runs.

This step is complete when you see ✔ Finished!

Two buttons will be presented at the completion of each run:

• Click View Results to launch a shiny app for interactively viewing results, or click Start new analysis to reset the app for another run.

Advanced: Results are organized under shiny_results/output/ in a run-specific folder named by timestamp with subfolders for gsea and ora results, each of which includes a folder of plots. Results include TSV and XLSX tables of enriched terms and pathway, as well as RDS versions of the complete input and output for each clusterProfiler run and a log file. Plots include volcano, dot, cnet, heatmap, upset, and more.

A second shiny app is allows you to interactively explore results and download individual files. Its side panel is organized in the same way as the output folders.

To relaunch the results app at any time, navigate to the top-level directory and

• Copy/paste these lines into the Console and hit return:

options(shiny.launch.browser = .rs.invokeShinyWindowExternal)
source("view_results.R")

For a given run, dataset and method (see side panel), the Data tab displays the processed input data as a table (left) and volcano plot (right). The table is paginated and supports search, filtering and download as Excel, CSV or to clipboard. The volcano plot uses the user-specified fold.change and p.value cutoffs (for ORA) and can also be downloaded. A checkbox near the top of the panel allows you to also view the genes that were excluded from the analysis, e.g., due to failed identifier mapping.

Pro-tip: View the excluded data rows to assess whether the exclusions are resolvable (e.g., fixing identifiers in your dataset CSV) and substantial (e.g., a lot of significant genes per the volcano plot).

Volcano plot

Using EnhancedVolcano, a volcano plot of your input data highlights subsets of genes based on the p.value and fold-change cutoffs indicated during the run. Plot options includes:

• Top n genes - How many genes to label in the plot (ignored if genes names are entered)
• Enter genes by name - A list of gene names with auto-populated choices from the top 20 (overrides Top n genes)
• Label font size - Font size for gene labels
• Legend position - Placement of the legend
  
  
  
  

Bar plot (Data)

Using ggplot2::geom_bar, a bar plot of your input data highlights positive and negative fold-change values for a subset of genes. Plot options includes:

• Top n genes - How many genes to include in the plot (ignored if genes names are entered)
• Enter genes by name - A list of gene names with auto-populated choices from the top 20 (overrides Top n genes)
• Label font size - Font size for all text
• Legend position - Placement of the legend
  
  
  
  
  

Per selected database (see the top drop-down menu), the Results tab displays a table of enriched terms and pathways, a plot of top results (left), and a plot of a selected result (right) from the table (above). The table is paginated and supports selection, search, filtering and download as Excel, CSV or to clipboard. The plots for top results include dot (by Gene Ratio or by Count), emap, concept networks, etc., and can also be downloaded. The plots for individual, selected results include GSEA score (for GSEA), database-specific views and linkouts, and more, and can also be downloaded.

Pro-tip: Navigate between GSEA and ORA methods (side panel) while viewing the results for a particular database to explore hits in common (and unique) to the different methods.

Dot plot

Using enrichplot, a dot plot of results highlight multiple statistics for the most enriched results. Plot options includes:

• Top n results - How many results to include in the plot
• X-axis by - Set the x-axis by Count (number of dataset genes overlapping a given term) or GeneRatio (dataset genes overlapping a given term vs all possible terms)
• Size by - Set the size of dots by Count, GeneRatio, or Percentage (dataset genes overlapping a given term vs all genes in term)
• Wrap labels - Wrap long labels to mulitple lines after a specified number of characters
• Label font size - Font size for all text
• Color by - Set the color of dots by pvalue, p.adjust, or qvalue (see clusterProfiler docs)
  
  
  
  
  

Bar plot (Results)

Using ggplot2::geom_bar, a bar plot of results highlight various statistics for the most enriched results. Plot options includes:

• Top n results - How many results to include in the plot
• X-axis by - Set the x-axis by Fold change (average fold change of overlapping genes), Count (number of dataset genes overlapping a given term) or Percentage (dataset genes overlapping a given term vs all genes in term)
• Wrap labels - Wrap long labels to mulitple lines after a specified number of characters
• Label font size - Font size for all text
  
  
  
  

Heatmap

Using ggplot2::geom_tile, a heatmap of results highlights overlapping genes among the most enriched results. Plot options includes:

• Top n results - How many results to include in the heatmap analysis (will exclude results without shared genes)
• Top n genes - How many genes to include in the heatmap, ordered by number of shared results and secondarily by fold.change.
• Wrap labels - Wrap long labels to mulitple lines after a specified number of characters
• Label font size - Font size for result labels (y-axis)
• Gene font size - Font size for gene labels (x-axis)
  
  
  

Enrichment map (emap)

Using enrichplot, an enrichment map connects results with overlapping genes. Plot options includes:

• Top n results - How many results to include in the heatmap analysis (will exclude results without shared genes)
• Wrap labels - Wrap long labels to mulitple lines after a specified number of characters
• Label font size - Font size for node labels
• Result node scale - Relative scale factor for the size of nodes
• Color by - Set the color of dots by pvalue, p.adjust, or qvalue (see clusterProfiler docs)
• Layout - Layout algorithm to apply to network. Layout names are rarely descriptive, so just try them out!
  
  
  
  
  

Concept network (cnet)

Using enrichplot, a concept network connects results via their overlapping genes. Plot options includes:

• Top n results - How many results to include in the heatmap analysis (will exclude results without shared genes)
• Label font size - Font size for category labels
• Gene font size - Font size for gene nodes
• Result node scale - Relative scale factor for the size of result nodes
• Gene node scale - Relative scale factor for the size of gene nodes
• Layout - Layout algorithm to apply to network. Layout names are rarely descriptive, so just try them out!
• Circular - Force a circular layout (pretty useless)
• colorEdge - Color the connections by result
  
  
  
  

PDF export

All plots offer PDF options to control the aspect ratio and initial dimensions (in pixels) of the saved PDF image. PDF images, of course, are vector graphics, so you can zoom in to the final image as much as you want. These are publication-ready images.

You are free to use all the files produced by this tool in your research and publications. Again, an initial set of plots, tables and RDS files is automatically generated in shiny_result/output/… and you can also download individual tables and plots from the interactive app.

In addition, the project is structured to facilitate sharing your results along with the interactive app! All the necessary files are maintained under the shiny_result folder. Share this folder (e.g., as a zip file, Dropbox or Box), or upload it to a cloud-hosted service (e.g., shinyapps.io) and share a simply URL. This is the best way to publish your enrichment results, allowing other researchers to browse your full results in spite of the limited space in publications.

The databases provided here each deserve a citation if you decide to publish or share your enrichment analysis results.

1. Martens M, Ammar A, Riutta A, Waagmeester A, Slenter DN, Hanspers K, Miller RA, Digles D, Lopes EN, Ehrhart F, Dupuis LJ, Winckers LA, Coort SL, Willighagen EL, Evelo CT, Pico AR, Kutmon M. WikiPathways: connecting communities Nucleic Acids Research, (2021), Volume 49, Issue D1, 8 January 2021, Pages D613--D621, doi: 10.1093/nar/gkaa1024
2. Hanspers K, Riutta A, Summer-Kutmon M, Pico AR*.* Pathway information extracted from 25 years of pathway figures. Genome Biol 21, 273 (2020). doi: 10.1186/s13059-020-02181-2
3. Gene Ontology Consortium. The Gene Ontology resource: enriching a GOld mine. Nucleic Acids Res. 2021;49(D1):D325-D334. doi: 10.1093/nar/gkaa1113

The enrichment analysis implementations were implemented by Guangchuang Yu in the clusterProfiler R package. Please cite:

Wu T, Hu E, Xu S, Chen M, Guo P, Dai Z, Feng T, Zhou L, Tang W, Zhan L, Fu x, Liu S, Bo X, Yu G (2021). clusterProfiler 4.0: A universal enrichment tool for interpreting omics data. The Innovation, 2(3), 100141. doi: 10.1016/j.xinn.2021.100141.

Many of the visualizations of enrichment results were also implemented by Guangchuang Yu in the enrichPlot R package. Please cite:

Yu G (2022). enrichplot: Visualization of Functional Enrichment Result. R package version 1.18.1, >online book.

The interactive Shiny applications used to setup, launch and view the results of enrichment analyses was developed by the Gladstone Institutes Bioinformatics Core. Please cite this repo and consider acknowledging our group if these tools prove useful.

https://github.com/gladstone-institutes/Interactive-Enrichment-Analysis

We thank the Gladstone Bioinformatics Core for their interactive enrichment analysis tools.

1. Error at launch

  Error in runUrl(url, subdir = subdir, destdir = destdir, ...) :
  Failed to download URL https://github.com/gladstone-institutes/Interactive-Enrichment-Analysis/archive/HEAD.tar.gz

• Solution: Your working directory may be set to a read-only dir, e.g., "/". Try setting it to your user dir.

2. Error running GSEA

  Warning: Error in preparePathwaysAndStats: Not all stats values are finite numbers

• Solution: You may have invalid p.values, e.g., zero is not an acceptable p.value. Replace these cases with a p.value smaller than any other in your dataset, then try again.

3. Dataset not working or GSEA not offered as option

• Solution: Double check that your columns names are exactly: gene fold.change and p.value. Other names will not be recognized by the tool.

4. Error running ORA

Error in <Anonymous>: 'geneSets' is not a slot in class "NULL"

• There are no genes that pass your significance filter criteria and ID mapping to Entrez ID. Explore your dataset in Excel to choose alternative criteria, e.g., larger p.value and/or smaller fold.change.