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clintval avatar clintval commented on June 24, 2024 1

Thanks for the idea @ElDeveloper!

Did you know that you can make additional arbitrary key-value pairs in the [Header] section of the sample sheet and even encode all your metadata in the sample sheet proper? I would recommend doing that so all your information is visible to the reader of the sample sheet. Although the file specification allows lines that start with a #, these would show up in a CSV file as the first data field in a row starting with a # and would not be parsed as a comment.

The Illumina specification on this section:

The Header section is required, and must be located on the first line of the Sample Sheet file. The Header contains informational fields describing the context around which a sequencing run or analysis was performed (eg, date, workflow, library prep kit, chemistry, etc.).

Header records are represented as a series of key-value pairs. As such, each line requires exactly two fields. The first field in each line is the "key," which names the piece of metadata being recorded. Each key in the Header section must be unique.
The second field in each line is the "value," which is the actual piece of metadata being recorded. Values do not necessarily need to be unique.

Example of a legal "Header" section containing records describing "Date" and "Investigator":

[Header]
Date,2007-01-26
Workflow,GenerateFASTQ
Investigator,John Smith

Example of a legal "Header" section (with padded commas):

[Header],,,,,,
Date,2007-01-26,,,,,
Workflow,GenerateFASTQ,,,,,
Investigator,John Smith,,,,,

https://www.illumina.com/content/dam/illumina-marketing/documents/products/technotes/sequencing-sheet-format-specifications-technical-note-970-2017-004.pdf

from sample-sheet.

ElDeveloper avatar ElDeveloper commented on June 24, 2024

Fair enough, thanks for the reply. I agree with you, moving "comments" to a dedicated section would likely be the best.

from sample-sheet.

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