Following instructions with correct Ubuntu and Nextflow versions produces this (excerpted) error:
Error: package or namespace load failed for ‘DESeq2’ in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]):
namespace ‘xfun’ 0.15 is being loaded, but >= 0.19 is required
In addition: Warning message:
package ‘matrixStats’ was built under R version 3.6.3
Execution halted
Pipeline completed with errors-
WARN: To render the execution DAG in the required format it is required to install Graphviz -- See http://www.graphviz.org for more info.
Error executing process > 'CONSENSUS_PEAKS_DESEQ2 (SPT5)'
Caused by:
Process `CONSENSUS_PEAKS_DESEQ2 (SPT5)` terminated with an error exit status (1)
Command executed:
featurecounts_deseq2.r \
--featurecount_file SPT5.consensus_peaks.featureCounts.txt \
--bam_suffix '.mLb.clN.bam' \
--outdir ./ \
--outprefix SPT5.consensus_peaks \
--outsuffix '' \
--cores 2 \
sed 's/deseq2_pca/deseq2_pca_1/g' <deseq2_pca_header.txt >tmp.txt
sed -i -e 's/DESeq2 /SPT5 DESeq2 /g' tmp.txt
cat tmp.txt SPT5.consensus_peaks.pca.vals.txt > SPT5.consensus_peaks.pca.vals_mqc.tsv
sed 's/deseq2_clustering/deseq2_clustering_1/g' <deseq2_clustering_header.txt >tmp.txt
sed -i -e 's/DESeq2 /SPT5 DESeq2 /g' tmp.txt
cat tmp.txt SPT5.consensus_peaks.sample.dists.txt > SPT5.consensus_peaks.sample.dists_mqc.tsv
find * -type f -name "*.FDR0.05.results.bed" -exec echo -e "bwa/mergedLibrary/macs/broadPeak/consensus/SPT5/deseq2/"{}"\t255,0,0" \; > SPT5.consensus_peaks.igv.txt
Command exit status:
1
Command output:
(empty)
Command error:
dirname, do.call, duplicated, eval, evalq, Filter, Find, get, grep,
grepl, intersect, is.unsorted, lapply, Map, mapply, match, mget,
order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank,
rbind, Reduce, rownames, sapply, setdiff, sort, table, tapply,
union, unique, unsplit, which, which.max, which.min
Attaching package: ‘S4Vectors’
The following object is masked from ‘package:base’:
expand.grid
Loading required package: IRanges
Loading required package: GenomicRanges
Loading required package: GenomeInfoDb
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: DelayedArray
Loading required package: matrixStats
Attaching package: ‘matrixStats’
The following objects are masked from ‘package:Biobase’:
anyMissing, rowMedians
Loading required package: BiocParallel
Attaching package: ‘DelayedArray’
The following objects are masked from ‘package:matrixStats’:
colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges
The following objects are masked from ‘package:base’:
aperm, apply, rowsum
Error: package or namespace load failed for ‘DESeq2’ in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]):
namespace ‘xfun’ 0.15 is being loaded, but >= 0.19 is required
In addition: Warning message:
package ‘matrixStats’ was built under R version 3.6.3
Execution halted
Work dir:
/home/j/chipseq_20210330/data/work/4e/c7a9b7394c7760781354adda99f45c
Tip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line