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Final diagnostics on OTU accumulation

Remember to do the final diagnostics (which you should have done to start with!)

  • Plot sequence abundance vs richness. Whatever the outcome, you may have to explain it if there is a relationship. The lines may look different among field types
  • Produce rarefaction curves for each site. Look for outliers or obvious evidence that we have significantly undersampled. Do this with the set of 6 samples per field, and/or with all the samples we have.

# s axis sequence abundance, y axis richness

Fit years since with `envfit()`

Remember to produce this plot and test in the microbial communities script. It isn't a constrained anaylsis; those will be done later with environmental variables. But this is a quick way to get a naive test on years since restoration, and it's likely to be significant with all microbial datasets.

#' Try fitting years since restoration with envfit...

NA point in ITS PCoA

Now for some reason one of the remnants is NA. I need to re-run all the code and troubleshoot. This is endless.

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