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jharenza avatar jharenza commented on July 28, 2024 1

Ok, yeah once we merge, I can just separate into two RDS files.

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jaclyn-taroni avatar jaclyn-taroni commented on July 28, 2024

@jharenza we may want to split the expression data files up based on the RNA library based on @jashapiro 's results on #120 - notebook here

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jharenza avatar jharenza commented on July 28, 2024

ok - separate RDS files for FPKM from each algorithm, as well as counts?

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jaclyn-taroni avatar jaclyn-taroni commented on July 28, 2024

That seems like it would be the most flexible way to go as far as downstream options if it's straightforward to do.

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jharenza avatar jharenza commented on July 28, 2024

@jharenza we may want to split the expression data files up based on the RNA library based on @jashapiro 's results on #120 - notebook here

Thinking about this again - would it be easier for those working on RNA to do this separation using the clinical file? I ask because many other analyses using these files may have to re-combine (eg - fusion workflow will use both stranded and polyA and if we look for CNV deletion evidence on the basis of RNA expression <1 FPKM, we would recombine these files). I agree we should not cluster/perform certain analyses together, but trying to think of what makes more sense - for those specific analyses tainted by strand to separate or for us to separate all now and make sure other users know they may have to re-combine. Thoughts?

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jashapiro avatar jashapiro commented on July 28, 2024

I would still support separating the files, and having the users combine them as needed. This means that the user will have to explicitly acknowledge that they are combining two different data sets, and may give a slight bit of pause to consider whether that is appropriate. For example, the meaning of FPKM < 1 is likely to be different in the two data sets, and the using the same cutoffs may not be appropriate.

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jharenza avatar jharenza commented on July 28, 2024

@jashapiro - makes sense! We will plan on separating them then.

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jharenza avatar jharenza commented on July 28, 2024

@cgreene @jaclyn-taroni @jashapiro - we have the CHANGELOG and all files except the transcript counts file staged and ready to go - that merge is taking longer than anticipated, so @yuankunzhu will either create the PR without that file later tonight and add tomorrow or just create the whole PR tomorrow morning.

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