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BLAST_BulkParse

Simple script to parse bulk BLAST results, selecting single best match, then classifying as good or bad hit

Dependencies

  1. Python 3 installation
  2. pandas module: pip install pandas

Instructions:

  1. take a fasta with multiple queries (ie, multiple fasta seqs in a single file)

  2. BLAST against a database using the following arguments. Modify -db, -query, and -num_threads as needed.
    blastn -task megablast -db combined_ITS_db.fasta -query bulk_queries.fasta -num_threads 12 -outfmt "6 qseqid sscinames sacc stitle qstart qend qlen length pident mismatch gaps evalue bitscore staxids" -out blastTable.tsv

  3. Run this script to parse results:
    BLAST_BulkParse.py --input blastTable.tsv --output bestHitsTable.tsv

  4. Three resulting files: all hits, good hits (>=97 pident) , and bad hits (<97 pident)

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