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Vahadane论文normalization

Implementation of "Structure-Preserving Color Normalization and Sparse Stain Separation for Histological Images (Vahadane normalization)"

实验报告:https://github.com/xindubawukong/Vahadane/blob/master/report.pdf

简要说明

代码支持python3,未进行2、3兼容检查。

核心代码在vahadane.py

需要额外安装的库有:spams, opencv。我spams安装的时候遇到一些问题,但是因为我的mac的原因。直接使用pip安装spams。opencv可自己找相应操作系统下的教程安装。

如果你装了jupyter notebook,可以运行一下main.ipynb,这里面是一个写好了的demo。

Report.md是我写给学姐看的,分析了一下参数选择的效果。可以不看。

使用方法

1 首先调用utils.py里的read_image函数读取图片。这个函数将输入图片从opencv默认的BGR转成RGB,并且对整体颜色进行了一些加强。读出来的图像矩阵是$nm3$的。

2 新建一个vahadane对象。两个LAMBDA已经调到最优。fast_mode是是否快速分解,getH_mode也会影响分解速度。ITER是getW时的一个参数。可以都试一下看看效果。

vhd = vahadane(LAMBDA1=0.01, LAMBDA2=0.01, fast_mode=1, getH_mode=0, ITER=50)
vhd.show_config()

3 矩阵分解

Ws, Hs = vhd.stain_separate(source_image)
vhd.fast_mode=0;vhd.getH_mode=0;  # 在获取target的分解时,为了更精确,不用fast模式
Wt, Ht = vhd.stain_separate(target_image)

4 合成target

img = vhd.SPCN(source_image, Ws, Hs, Wt, Ht)

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