Comments (1)
Hi @sunsetyerin,
Nanocompore is limited to 2 clusters for the gmm. If 1 cluster fits the data better than 2, no further processing is done and the site is considered unmodified. If 2 clusters fit the data better than 2, the statical test (usually the logistical regression test) is performed.
From this example there are 32 reads from control 1 assigned to cluster 1 (c1) and 12 reads assigned to cluster 2 (c2). Control 2 has 21 reads assigned to c1 and 9 reads assigned to c2. Test 1 has 60 reads assigned to c1 and 23 reads assigned to c2, and test 2 has 38 reads assigned to c1 and 16 reads assigned to c2.
The basic way to read those lines is:
[sample name]:[number of reads assigned to cluster 1]/[number of reads assigned to cluster 2]__repeated for each further sample.
I hope this explanation helps.
from nanocompore.
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from nanocompore.