A clinical genomics-guided prioritizing strategy enables accurately selecting proper cancer cell lines for biomedical research
Selecting appropriate cell lines to represent a disease is crucial for the success of biomedical research, since the usage of less relevant cell lines could bring misleading results. However, no systematic guidance is available yet for the cell lines selection. Here we developed a clinical Genomics-guided Prioritizing Strategy for Cancer Cell Lines (CCL-cGPS) to sort cell lines. Statistical analyses revealed CCL-cGPS selected cell lines were among the most appropriate models. Moreover, we observed a linear correlation between the drug response and CCL-cGPS score of cell lines for breast and thyroid cancers. Using CCL-cGPS selected RT4 cells, we identified mebendazole and digitoxin as candidate drugs against bladder cancer, and validate their promising anticancer effect through in vitro and in vivo experiments. Additionally, a web tool was developed. CCL-cGPS bridges the gap between tumors and cell lines, presenting a helpful guide to select the most suitable cell line models.
For each tumor subtype, CCL-cGPS suggests several cancer cell lines that mimic clinical tumor patients.
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Bladder cancer (BLCA)
- Non-papillary:
SCaBER
,HT-1197
,UM-UC-3
,SW-1710
,VM-CUB1
- Papillary:
RT4
(commonly-used),VM-CUB1
,RT112_84
,SW_780
- Non-papillary:
-
Breast cancer (BRCA)
- Infiltrating ductal:
HCC2218
,HCC1428
,HCC2157
,HCC1599
,ZR-75-30
- Infiltrating lobular:
UACC-812
,CAL-148
,YMB-1
,ZR-75-1
,ZR-75-30
- ER+:
HCC2218
,HCC1428
,ZR-75-30
,MDA-MB-134-VI
,CAL-148
- HER2+:
SK-BR-3
(commonly-used),HCC202
- Triple negative:
HCC1599
,HCC2157
,HCC1569
,CAL-85-1
,HCC1143
- Infiltrating ductal:
-
Bile duct cancer (CHOL)
- Intrahepatic:
SNU-245
- Intrahepatic:
-
Colorectal cancer (COADREAD)
- Colon adenocarcinoma (COAD):
NCI-H747
,LS123
,SW1417
,RCM-1
,C2BBe1
- Colon mucinous adenocarcinoma:
CL-11
,Hs_675_T
,MDST8
- Rectal adenocarcinoma (READ:
NCI-H747
,SW1417
,COLO-678
,SW1463
,RCM-1
(commonly-used) - KRAS mutated:
HCC-56
,LS123
,SW1463
,SW948
,COLO-678
- Colon adenocarcinoma (COAD):
-
Esophagus cancer (ESCA)
- Esophagus adenocarcinoma:
OE19
,KYSE-270
,OE33
(commonly-used),TE-1
- Esophagus squamous cell carcinoma:
COLO-680N
,KYSE-140
,TE-9
,T_T
- Esophagus adenocarcinoma:
-
Glioma
-
Head and neck squamous cell carcinoma (HNSC)
- HNSC:
BICR_31
,SCC-4
,PE_CA-PJ34_clone_C12
,BICR_18
,PE_CA-PJ41_clone_D2
- HNSC:
-
Kidney cancer
- Kidney chromophobe (KICH):
KMRC-3
,SNU-349
,VMRC-RCW
,ACHN
- Kidney renal clear cell carcinoma (KIRC):
A-704
,KMRC-3
,SNU-349
,OSRC2
,TUHR4TKB
- Kidney renal papillary cell carcinoma (KIRP):
A-704
,Caki-1
,VMRC-RCW
,ACHN
(commonly-used),OSRC2
- Kidney chromophobe (KICH):
-
Liver cancer
- Liver hepatocellular carcinoma (LIHC):
SNU-449
,SNU-878
,Alexander_cells
,SK-HEP-1
,JHH-1
- Liver hepatocellular carcinoma (LIHC):
-
Lung cancer
-
Mesothelioma (MESO)
- Epithelioid:
JL-1
,IST-MES1
- Biphasic:
NCI-H2052
- Epithelioid:
-
Ovarian cancer (OV)
- OV:
OVSAHO
,JHOS-2
,COV362
,KURAMOCHI
,OAW28
- OV:
-
Pancreatic cancer (PAAD)
- PAAD:
QGP-1
,PANC-1
(commonly-used),SW_1990
,KCI-MOH1
- PAAD:
-
Prostatic cancer (PRAD)
- PRAD:
VCaP
,PC-3
(commonly-used),NCI-H660
,22Rv1
- PRAD:
-
Melanoma (SKCM
- SKCM:
COLO_792
,RVH-421
,MEL-HO
,Hs_839_T
,Malme-3M
- SKCM:
-
Stomach cancer (STAD)
- Diffuse:
GCIY
,NUGC-3
,NUGC-4
- Mucinous:
ECC10
,HuG1-N
,KE-39
,MKN1
,RERF-GC-1B
- Tubular:
NCI-N87
,NUGC-4
,SH-10-TC
,ECC10
- Diffuse:
-
Thyroid cancer (THCA)
- Classical/usual:
TT
,FTC238
,ML-1
,FTC-133
- Follicular:
TT
,FTC-133
(commonly-used)),FTC238
- Tall cell:
CGTH-W-1
- Classical/usual:
-
Endometrium cancer
CCL-cGPS manuscript is under review. For more information, please visit our website tcm.zju.edu.cn/cgps. Should you have any questions, please contact Xin Shao at [email protected]