The MethPipe software package is a computational pipeline for analyzing bisulfite sequencing data (WGBS and RRBS). MethPipe provides tools for mapping bisulfite sequencing read and estimating methylation levels at individual cytosine sites. Additionally, MethPipe also includes tools for identifying higher-level methylation features, such as hypo-methylated regions (HMR), partially methylated domains (PMD), hyper-methylated regions (HyperMR), and allele-specific methylated regions (AMR).

You may download the latest stable release from:http://smithlabresearch.org/software/methpipe/
This software package has been designed to operate in a UNIX-like environment. It has been tested on MacOS X Snow Leopard and Linux.

• Step 0

  This software package requires a functioning installation of the GNU Scientific Library (GSL). If you don't already have this installed, you will need to download and install it from http://www.gnu.org/software/gsl/

• Step 1

  To build the binaries, type the following, where '>' is your prompt and the CWD is the root of the distribution:

  > make all
  

• Step 2

  To install the binaries, type the following, where '>' is your prompt and the CWD is the root of the distribution:

  > make install
  

  This will place the binaries in the bin directory under the package root. They can be used directly from there without any additional steps. You can add that directory to your PATH environment variable to avoid having to specify their full paths, or you can copy the binaries to another directory of your choice in your PATH

For advanced users who are interested in the newest features, you may obtain the latest source code by cloning the MethPipe repository:

> git clone --recursive https://github.com/smithlabcode/methpipe.git

After you clone the latest source code, follow the above steps for installation.

Read methpipe-manual.pdf in the docs directory.

Andrew D. Smith [email protected]

Qiang Song [email protected]

MethPipe and MethBase Users' Mailinglist [email protected] http://groups.google.com/group/methpipe?hl=en

Copyright (C) 2013 University of Southern California, Andrew D. Smith, Qiang Song, Fang Fang, Ben Decato and Meng Zhou

Authors: Andrew D. Smith, Qiang Song, Fang Fang, Ben Decato and Meng Zhou

This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program. If not, see http://www.gnu.org/licenses/.

SamTools Copyright (c) 2008-2009 Genome Research Ltd. SamTools software is free software distributed under the MIT License. Refer to the COPYING file in src/samtoos/ for further information.