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atena's Introduction

atena: analysis of transposable elements in R and Bioconductor

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Current Bioconductor build status

  • release Bioconductor Availability Bioconductor Dependencies Bioconductor Commits Bioconductor Release Build
  • development Bioconductor Availability Bioconductor Dependencies Bioconductor Commits Bioconductor Devel Build

The atena package provides access from R/Bioconductor to the following methods for the quantification of transposable element (TE) expression:

  • Jin Y et al. TEtranscripts: a package for including transposable elements in differential expression analysis of RNA-seq datasets. Bioinformatics. 2015;31(22):3593-3599. DOI: 10.1093/bioinformatics/btv422.

  • Tokuyama M et al. ERVmap analysis reveals genome-wide transcription of human endogenous retroviruses. PNAS. 2018;115(50):12565-12572. DOI: 10.1073/pnas.1814589115.

  • Bendall et al. Telescope: characterization of the retrotranscriptome by accurate estimation of transposable element expression. PLOS Comp. Biol. 2019;15(9):e1006453. DOI: 10.1371/journal.pcbi.1006453.

Installation

atena has been submitted to Bioconductor.

To install the release version of this package please go to its package release landing page at https://bioconductor.org/packages/atena and follow the instructions there to install it.

This github repository contains the development version of the R/Bioconductor package atena. This version is unstable and should be used only to test new features. To install the development version you first need to install the development version of Bioconductor and then type the following line from the R shell:

BiocManager::install("atena", version="devel")

Alternatively, you can directly install it from this GitHub repo as follows:

BiocManager::install("rcastelo/atena")

Questions, bug reports and issues

For questions and bug reports regarding the release version of atena please use the Bioconductor support site. For bug reports and issues regarding this development version of atena please use the GitHub issues tab at the top-left of this page.

Contributing

Contributions to the software codebase of atena are welcome as long as contributors abide to the terms of the Bioconductor Contributor Code of Conduct. If you want to contribute to the development of GenomicScores please open an issue to start discussing your suggestion or, in case of a bugfix or a straightforward feature, directly a pull request.

atena's People

Contributors

beacalvo avatar rcastelo avatar pablo-rodr-bio2 avatar jwokaty avatar nturaga avatar petehaitch avatar

Stargazers

Davide Biganzoli avatar  avatar CWCWW avatar Emeline Favreau avatar Ravindra Raut avatar Elsa Quezada avatar

Watchers

James Cloos avatar  avatar  avatar

atena's Issues

baySeq.build failure

biocbuild@kunpeng1 ~/git> R CMD build --keep-empty-dirs --no-resave-data baySeq
* checking for file ‘baySeq/DESCRIPTION’ ... OK
* preparing ‘baySeq’:
* checking DESCRIPTION meta-information ... OK
* installing the package to build vignettes
* creating vignettes ... ERROR
--- re-building ‘baySeq.Rnw’ using Sweave
Loading required package: GenomicRanges
Loading required package: stats4
Loading required package: BiocGenerics

Attaching package: ‘BiocGenerics’

The following objects are masked from ‘package:stats’:

    IQR, mad, sd, var, xtabs

The following objects are masked from ‘package:base’:

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call, duplicated,
    eval, evalq, get, grep, grepl, intersect, is.unsorted, lapply, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank, rbind,
    rownames, sapply, setdiff, sort, table, tapply, union, unique, unsplit,
    which.max, which.min

Loading required package: S4Vectors

Attaching package: ‘S4Vectors’

The following objects are masked from ‘package:base’:

    I, expand.grid, unname

Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: abind
Loading required package: parallel
Finding priors...done.
Length of priorReps:1000
Length of priorSubset:1000
Length of subset:1000
Length of postRows:1000
Finding priors...done.
Finding posterior likelihoods...Length of priorReps:0
Length of priorSubset:3000
Length of subset:3000
Length of postRows:3000
Analysing part 1 of 1
Preparing data...........................................................done.
Estimating likelihoods......done!
done.
Warning in summarisePosteriors(cD) :
  No orderings contained in countData object.
Finding priors...done.
Finding posterior likelihoods...Length of priorReps:0
Length of priorSubset:3000
Length of subset:3000
Length of postRows:3000
Analysing part 1 of 1
Preparing data.........................................................done.
Estimating likelihoods......done!
done.
--- finished re-building ‘baySeq.Rnw’

--- re-building ‘baySeq_generic.Rnw’ using Sweave
Finding priors...done.
Finding posterior likelihoods...Length of priorReps:0
Length of priorSubset:1000
Length of subset:1000
Length of postRows:1000
Analysing part 1 of 1
Preparing data..........................................................done.
Estimating likelihoods......done!
Warning in makeOrderings(listPosts[[cc]]) :
  No valid ordering function available.
done.
Finding priors...done.
Finding posterior likelihoods...Length of priorReps:0
Length of priorSubset:1000
Length of subset:1000
Length of postRows:1000
Analysing part 1 of 1
Preparing data....................................................done.
Estimating likelihoods......done!
Warning in makeOrderings(listPosts[[cc]]) :
  No valid ordering function available.
done.
Finding priors...done.
Finding posterior likelihoods...Length of priorReps:0
Length of priorSubset:1000
Length of subset:1000
Length of postRows:1000
Analysing part 1 of 1
Preparing data......................................................done.
Estimating likelihoods......done!
done.
Finding priors...done.
Finding posterior likelihoods...Length of priorReps:0
Length of priorSubset:1000
Length of subset:1000
Length of postRows:1000
Analysing part 1 of 1
Preparing data............................................................done.
Estimating likelihoods......done!
done.

Error: processing vignette 'baySeq_generic.Rnw' failed with diagnostics:
 chunk 20 
Error in pET %in% c("iteratively", "BIC") && (any(sapply(modelPriorValues,  : 
  'length = 2' in coercion to 'logical(1)'

--- failed re-building ‘baySeq_generic.Rnw’

SUMMARY: processing the following file failed:
  ‘baySeq_generic.Rnw’

Error: Vignette re-building failed.
Execution halted

"R CMD check" failure on Linux ARM64

Hello,

There is an initiative to support Linux ARM64 for Bioconductor.
While testing the packages we faced this failure:

biocbuild@kunpeng1 ~/git> R CMD check atena_1.5.10.tar.gz 
* using log directory ‘/home/biocbuild/git/atena.Rcheck’
* using R Under development (unstable) (2023-03-12 r83975)
* using platform: aarch64-unknown-linux-gnu (64-bit)
* R was compiled by
    gcc (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0
    GNU Fortran (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0
* running under: Ubuntu 22.04.2 LTS
* using session charset: UTF-8
* checking for file ‘atena/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘atena’ version ‘1.5.10’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘atena’ can be installed ... OK
* checking installed package size ... OK
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking startup messages can be suppressed ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking installed files from ‘inst/doc’ ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... ERROR
Running examples in ‘atena-Ex.R’ failed
The error most likely occurred in:

> ### Name: annotaTEs
> ### Title: Get RepeatMasker UCSC annotations
> ### Aliases: annotaTEs
> 
> ### ** Examples
> 
> rmsk_gr <- annotaTEs(genome = "hg19", parsefun = rmskidentity)
snapshotDate(): 2023-03-21
loading from cache
Error: failed to load resource
  name: AH99002
  title: UCSC RepeatMasker annotations (Mar2020) for Human (hg19)
  reason: error reading from connection
Execution halted
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘runTests.R’
 ERROR
Running the tests in ‘tests/runTests.R’ failed.
Last 13 lines of output:
  1 Test Suite : 
  atena RUnit Tests - 13 test functions, 0 errors, 1 failure
  FAILURE in test_TEtranscripts: Error in checkEqualsNumeric(head(sort(assay(ttSE), decreasing = TRUE)),  : 
    Mean relative difference: 0.007352941
  
  
  Test files with failing tests
  
     test_TEtranscripts.R 
       test_TEtranscripts 
  
  
  Error in BiocGenerics:::testPackage("atena") : 
    unit tests failed for package atena
  Execution halted
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in ‘inst/doc’ ... OK
* checking running R code from vignettes ...
  ‘atena.Rmd’ using ‘UTF-8’... OK
 NONE
* checking re-building of vignette outputs ... OK
* checking PDF version of manual ... OK
* DONE

Status: 2 ERRORs
See
  ‘/home/biocbuild/git/atena.Rcheck/00check.log’
for details.

Any idea why it fails ?
The check passes fine on x86_64 - https://bioconductor.org/checkResults/3.17/bioc-LATEST/atena but it fails consistently on aarch64.

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