A very simple class (subclass of tkinter.Label) that displays an animated GIF in a label and either runs the animation in a separate thread or using tkinter's after(). Written in Python3 and tested successfully on 2.7 (thanks to @brenden-t-r)

This allows using animated GIFs together with a normal tkinter mainloop() without blocking, but with caveats (see below) Perfect for showing small animated "please wait"/"working"/"downloading" icons while doing other tasks.

I recommend trying the non-threaded approach first. If you have stuttering in your animation, try the threaded approach

    from AnimatedGIF import *
  
    lbl_with_my_gif = AnimatedGif(parent, 'my_logo.gif', 0.04)  # (tkinter.parent, filename, delay between frames)
    lbl_with_my_gif.pack()  # Packing the label with the animated gif (grid works just as well)
    lbl_with_my_gif.start()  # Shows gif at first frame and we are ready to go
  		...
    lbl_with_my_gif.stop()  # Setting stop flag, which ends the update loop (animation)

    from AnimatedGIF import *
  
    lbl_with_my_gif = AnimatedGif(parent, 'my_logo.gif', 0.04)  # (tkinter.parent, filename, delay between frames)
    lbl_with_my_gif.pack()  # Packing the label with the animated gif (grid works just as well)
    lbl_with_my_gif.start_thread()  # Spawn thread which updates animation
  		...
    lbl_with_my_gif.stop_thread()  # Setting stop flag, which ends the animation

I made this after seeing a whole lot of questions on StackExchange on how to do this, but no real working solutions that allows for usage together with mainloop(), which I guess a lot of people, myself included, need.

If you are using the threaded aproach and get the "RuntimeError: main thread is not in main loop" error, you're experiencing a known problem with tkinter. Unfortunately, tkinter is not really thread safe. You can replace tkinter with mtTkinter, but in my experience, whether or not this becomes a problem varies from case to case. It seems that if your GIF animation thread makes the main tkinter thread time out, this error occurs. This isn't really unique to Tkinter, but more a general issues with gui frameworks in Python.

The "correct" way of using this class is to run a update() and update_idletasks() loop while the task you are waiting for run in a thread. This usually works better than putting the animation in a thread, at least if the task isn't making updates to the window.

Of course, the simple way of doing it is using the non-threaded stop and start methods, which uses an "after()-loop", but in some cases, especially where the program is busy, the animation can get very choppy, as after() does not guarantee timely execution. Which is fine in some cases and not in others. Use either approach as you see fit.

AnimateGIF is released under the MIT license (https://opensource.org/licenses/MIT)