Comments (5)
time used: 1 seconds
Seems that your files are very small?
Please check the downloaded files by comparing the MD5
from mutscan.
Here is my output
MacBook-Pro:MutScan shifu$ ./mutscan -1 R1.fq.gz -2 R2.fq.gz
---------------
NRAS-neg-1-115258748-2-c.34G>T-p.G12C-COSM562 GGATTGTCAGTGCGCTTTTCCCAACACCAC A TGCTCCAACCACCACCAGTTTGTACTCAGT chr1
1, pos: 166, distance: 0, reverse
@NB551106:59:HTFV3BGX2:1:11102:9413:4074 1:N:0:AGTCAA merged offset:78 overlap:71 diff:0
CCTCTTATTCCTTTAATACAGAATATGGGTAAAGATGATCCGACAAGTGAGAGACAGGATCAGGTCAGCGGGCTACCACTGGGCCTCACCTCTATGGTGGGATCATATTCATCTACAAAGTGGTTCTGGATTAGCT GGATTGTCAGTGCGCTTTTCCCAACACCAC A TGCTCCAACCACCACCAGTTTGTACTCAGT CATTTCACACCAGCAAGAACCTGTTGGAAACC
-
AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEeeiiieieiiiiiiieeieeeiiiiiiSiiieiiiieiiiiiiieiieeiiiiiii eiieiiiiiiiii``EEEEAEEEEEEEEEE E AEEEEEEEEEEEEEEEEE/EEEEEEEEEEE EEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA
2, pos: 80, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:13201:15696:12851 1:N:0:GATCAG merged offset:0 overlap:145 diff:0
CACCTCTATGGTGGGATCATATTCATCTACAAAGTGGTTCTGGATTAGCT GGATTGTCAGTGCGCTTTTCCCAACACCAC A TGCTCCAACCACCACCAGTTTGTACTCAGT CATTTCACACCAGCAAGAACCTGTTGGAAACCAG
-
eeeeeiieie`ieS`iieeiiiiiieeiiiiiiiiiiS`iiiiiiiiiii i`iiei`iiiiiiiiiiiiiiiiiiiiiii S iiiiiiieiiSiiiiiiiiiiiiiiiiiii ieiiieei`e`i`iiSSS`e`eSZeiiiieaaee
---------------
EGFR-pos-7-55249071-20-c.2369C>T-p.T790M-COSM6240 TCTGCCTCACCTCCACCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGCTGCCTCCTGGA chr7
1, pos: 132, distance: 1, forward
@NB551106:9:H5Y5GBGX2:1:21107:14365:13681 1:N:0:GATCAG
CTGGCCACCATGCGAAGCCACACTGACGTGCCTCTCCCTCCCTCCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCA TCTGCCTCACCTCCACCGTGCAGCTCATCA T GCAGCTCAGGCCCTTCGG
+
AAAAAEEEAAEEEAEEAEEEEEEEE/AEEEEEEEEEEEEEEEEEE<A<AAEEEEEEEEAEA<<EEEEEAEEE/E/AEEAEE/EEA/EEEAEEAA<AAEEEEA /EEEEE<E/EA<EA/E/EA/EE/<AEA<EE < A6/E<6<E/A/EAE/<</
2, pos: 102, distance: 1, reverse
@NB551106:9:H5Y5GBGX2:1:21107:14365:13681 2:N:0:GATCAG
CCTCTCCCTCCCTCCCGGATGCCTACGTGATGGCCAGCGTGGACATCCCCCACGTGTGCCGCCTGCTGGGCA TCTGCCTCACCTCCACCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGCTCCCTCCTGGA CTATGTCCGGGAACACAA
-
<</EEAA///AEA/</A<</E6<<//E/E6/AEE</<AE/EE/A//AEE/6/<E/EAEAAEEAEEA6EAAEE EEAEA/EEEAAEEA/AAEAEAAEE/E/EEE E AEA/EEEAEAEEAEEEEEEEEAEEEEEEEE EEEEEE/EEEEEE6A/AA
3, pos: 97, distance: 2, forward
@NB551106:9:H5Y5GBGX2:1:13107:23878:9922 1:N:0:GATCAG merged offset:0 overlap:121 diff:0
CCCTCCCTCCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCA TCTGCCTCACCTCCACCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGAAGCC
+
eeeaeiieieeS`SeeiieSi`ii`iiiiiiiiiSeiaieeiiiiieiieieiiiiiiiiieiiiii iiiieiai`ieiiiSiiieiiSiiiiSei` e iSeiiiiSiiiiie`iiiOOeeO
4, pos: 78, distance: 1, forward
@NB551106:9:H5Y5GBGX2:1:12302:13931:19052 1:N:0:GATCAG merged offset:29 overlap:122 diff:0
ACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCA TCTGCCTCACCTCCACCGTGCAACTCATCA T GCAGCTCATGCCCTTCGGCTGCCTCCTGGA CTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGG
+
AAAAAEEEEEEEEEEEEEEEEEEEEEEEEeSSiie`e`eii`SieeeO `eee`iiiOieii`Viii`SeOSiieiiee ` ie`iiiieiiiiiieiiiiiiiiiiii`iS SZSiieiiiiiSeiSiieieiSSi`e`eii`ZS``ei`eeiSEEEEEEEEEEEEEEEEEEEEEEEEAAAAA
5, pos: 60, distance: 2, reverse
@NB551106:9:H5Y5GBGX2:1:21312:26680:10752 1:N:0:GATCAG
CGCAGCTCCCCGTGTGCCGCCTGCTGGGCA TCTGCCTCACCTCCAGCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGCTCCCTCCTGGA CTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCA
-
///////<///A/E/E//E/AEE<AAA//< /A/EEEE///A</E///A</E/AE/EAE// E /66EE/EAEE/EEEAAEEEEEEEE/AEEE/ EEEEEEAE/AEEAE/EEE/E/E/EEAEEEE6EEE//EAE/EEAEEEAEEEAEEAEA6AAA
6, pos: 51, distance: 1, forward
@NB551106:9:H5Y5GBGX2:1:13201:25711:16931 2:N:0:GATCAG
ACGTGTGCCGCCTGCTGGGCA TCTGCCTCACCTCCACCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGCTCCCTCCTGGA CTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAA
+
AAAAAEEEEEEEEEEEEEEEE EEEEEEEEEEEEEEEEAAEEEEEAEEEEEE E EEEEEEEEEEEEEEEEEEEEEEEEEEEEEE EEEEEEEEEEAEEEAEEEAE/EEEAEEEEEEEEA/<EEEAEAEAEEEE<AA<A/AAEEAE//A<<EEE
7, pos: 48, distance: 1, reverse
@NB551106:9:H5Y5GBGX2:1:21310:7418:7755 1:N:0:GATCAG merged offset:0 overlap:125 diff:0
TGTGCCGCCTGCTGGGCA TCTGCCTCACCTCCACCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGCTCCCTCCTGGA CTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTC
-
aeOeeiieiiiiiieiii ieiiiiiiSiiiiiZiiiiiiiiiSiiiii i iieiiiiZiiiiiiiiiiieiiiiiiiiSe iiieSiSi`eiSeeSeiiiiSeZi``iiiiieiSeiiSOSie\eOe
8, pos: 48, distance: 2, reverse
@NB551106:9:H5Y5GBGX2:1:13201:25711:16931 1:N:0:GATCAG
GGTGCCGCCTGCTGGGCA TCTGCCTCACCTCCCCCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGCTCCCTCCTGGA CTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGT
-
<//6E<AEE<A<E<A<A< E/E<A</E/EAEEE/EEEEAE/EEEAEEEA E EA/EE/EEEEEEEE/EEEEEEEEEEE/EEE EEEEEEEEAEEEEEEEEEEEEE6EE/EEEEEEEEEEEEEEEEEE6EEEEEEEEE/E/EEEEEEEEEEAAAA/
9, pos: 26, distance: 2, reverse
@NB551106:9:H5Y5GBGX2:1:11207:19779:6631 1:N:0:GATCAG merged offset:0 overlap:149 diff:1
CCTCACCTCCACCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGAAGCCTCCTGGA CTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGTAATCAGGGAAGGGAGATACG
-
eeQa\ieiiOiaSVSZiiii`iOiii i iiiii`S`Siiiiiie`ie`iie`iSiiSe iiiieSiiieiSSSSiiiieZiiieiiSSiiiieieSeeiSeiieiiAiieiJiSiieieiSSeeO\SiiSiei`iie`SSeSeieiVVOOa
10, pos: 26, distance: 2, reverse
@NB551106:9:H5Y5GBGX2:1:11307:24343:19438 1:N:0:GATCAG merged offset:0 overlap:149 diff:0
CCTCACCTCCACCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGAAGCCTCCTGGA CTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGTAATCAGGGAAGGGAGATACG
-
\aa\VeiSe`\eiS`iieiiei`eeS i SiZSeieeiieSiS`iiiaSiiiiiiiSii iieiieiieiie`i`ieiiiSiiiiiiiiiiSiiieiiaeiiiiii`eiiiii`OiiiiiiSeiiiiiiiiiSeS`iie`iee`e`eOOaea
11, pos: 19, distance: 2, reverse
@NB551106:9:H5Y5GBGX2:1:11101:3081:15503 1:N:0:GATCAG merged offset:0 overlap:144 diff:2
TCCACCGTGCAGCTCATCA T GCAGCTCATGCCCTTCGGAAGCCTCCTGGA CTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGTAATCAGGGAAGGGAGATACGGG
-
OeAeaSi`eeVeSieiiii ` SeieSiei`iOJiiieeieiSiiiSiiiie iiiSiSieeSiSiiiiiiii`ieeiiiieeieZiZZiiiiiiee\ZeiiiaiiiS``eiiiE`S`Sai`eieiS`eeieeiieSZeeieOVO\V
---------------
EGFR-pos-7-55249091-20-c.2389T>A-p.C797S-COSM5010368 CAGCTCATCACGCAGCTCATGCCCTTCGGC A GCCTCCTGGACTATGTCCGGGAACACAAAG chr7
1, pos: 46, distance: 2, reverse
@NB551106:9:H5Y5GBGX2:1:11207:19779:6631 1:N:0:GATCAG merged offset:0 overlap:149 diff:1
CCTCACCTCCACCGTG CAGCTCATCATGCAGCTCATGCCCTTCGGA A GCCTCCTGGACTATGTCCGGGAACACAAAG ACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGTAATCAGGGAAGGGAGATACG
-
eeQa\ieiiOiaSVSZ iiii`iOiiiiiiiii`S`Siiiiiie`ie ` iie`iSiiSeiiiieSiiieiSSSSiiiie ZiiieiiSSiiiieieSeeiSeiieiiAiieiJiSiieieiSSeeO\SiiSiei`iie`SSeSeieiVVOOa
2, pos: 46, distance: 2, reverse
@NB551106:9:H5Y5GBGX2:1:11307:24343:19438 1:N:0:GATCAG merged offset:0 overlap:149 diff:0
CCTCACCTCCACCGTG CAGCTCATCATGCAGCTCATGCCCTTCGGA A GCCTCCTGGACTATGTCCGGGAACACAAAG ACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGTAATCAGGGAAGGGAGATACG
-
\aa\VeiSe`\eiS`i ieiiei`eeSiSiZSeieeiieSiS`iiia S iiiiiiiSiiiieiieiieiie`i`ieiii SiiiiiiiiiiSiiieiiaeiiiiii`eiiiii`OiiiiiiSeiiiiiiiiiSeS`iie`iee`e`eOOaea
3, pos: 39, distance: 2, reverse
@NB551106:9:H5Y5GBGX2:1:11101:3081:15503 1:N:0:GATCAG merged offset:0 overlap:144 diff:2
TCCACCGTG CAGCTCATCATGCAGCTCATGCCCTTCGGA A GCCTCCTGGACTATGTCCGGGAACACAAAG ACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGTAATCAGGGAAGGGAGATACGGG
-
OeAeaSi`e eVeSieiiii`SeieSiei`iOJiiieeie i SiiiSiiiieiiiSiSieeSiSiiiiiiii `ieeiiiieeieZiZZiiiiiiee\ZeiiiaiiiS``eiiiE`S`Sai`eieiS`eeieeiieSZeeieOVO\V
4, pos: 28, distance: 2, forward
@NB551106:9:H5Y5GBGX2:1:11210:13966:15731 2:N:0:GATCAG
GCTCATCATGCAGCTCATGCCCTTCGGA A GCCTCCTGGACTATGTCCGGGAACACAAAG ACAATATTGGCTCCCAGTACCTGCTCAACCGGTGTGTGCAGATCGCAAAGGTAATCAGGGAAGGGAGATACGGGGAGGGGAGAGTCGGAGCC
+
AAAAAEEEAEEAEEEEEEEEEEAEEEEE A EEEEEEEEEAEEEEEEEEEEEE/E6EEEEE //EAAEEEEEEEEEE<AE/EEEEAEE//EEE6A<EEEE/EE/<EEE/EEAE<E//EE<EE//6<<AE/E/<AA<A/EAA6A/A///6</6</
---------------
EGFR-pos-7-55249092-20-c.2390G>C-p.C797S-COSM5010368 AGCTCATCACGCAGCTCATGCCCTTCGGCT C CCTCCTGGACTATGTCCGGGAACACAAAGA chr7
1, pos: 123, distance: 1, reverse
@NB551106:9:H5Y5GBGX2:1:21107:14365:13681 2:N:0:GATCAG
CCTCTCCCTCCCTCCCGGATGCCTACGTGATGGCCAGCGTGGACATCCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACCGTGC AGCTCATCATGCAGCTCATGCCCTTCGGCT C CCTCCTGGACTATGTCCGGGAACACAA
-
<</EEAA///AEA/</A<</E6<<//E/E6/AEE</<AE/EE/A//AEE/6/<E/EAEAAEEAEEA6EAAEEEEAEA/EEEAAEEA/AAEAEA AEE/E/EEEEAEA/EEEAEAEEAEEEEEEE E AEEEEEEEEEEEEEE/EEEEEE6A/AA
2, pos: 81, distance: 1, reverse
@NB551106:9:H5Y5GBGX2:1:21312:26680:10752 1:N:0:GATCAG
CGCAGCTCCCCGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCAGCGTGC AGCTCATCATGCAGCTCATGCCCTTCGGCT C CCTCCTGGACTATGTCCGGGAACACAAAGA CAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCA
-
///////<///A/E/E//E/AEE<AAA//</A/EEEE///A</E///A</E /AE/EAE//E/66EE/EAEE/EEEAAEEEE E EEE/AEEE/EEEEEEAE/AEEAE/EEE/E/ E/EEAEEEE6EEE//EAE/EEAEEEAEEEAEEAEA6AAA
3, pos: 72, distance: 1, forward
@NB551106:9:H5Y5GBGX2:1:13201:25711:16931 2:N:0:GATCAG
ACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACCGTGC AGCTCATCATGCAGCTCATGCCCTTCGGCT C CCTCCTGGACTATGTCCGGGAACACAAAGA CAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAA
+
AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEE EEAEEEEEEEEEEEEEEEEEEEEEEEEEEE E EEEEEEEEEEEEEEEEEEEAEEEAEEEAE/ EEEAEEEEEEEEA/<EEEAEAEAEEEE<AA<A/AAEEAE//A<<EEE
4, pos: 69, distance: 1, reverse
@NB551106:9:H5Y5GBGX2:1:21310:7418:7755 1:N:0:GATCAG merged offset:0 overlap:125 diff:0
TGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACCGTGC AGCTCATCATGCAGCTCATGCCCTTCGGCT C CCTCCTGGACTATGTCCGGGAACACAAAGA CAATATTGGCTCCCAGTACCTGCTC
-
aeOeeiieiiiiiieiiiieiiiiiiSiiiiiZiiiiii iiiSiiiiiiiieiiiiZiiiiiiiiiiie i iiiiiiiSeiiieSiSi`eiSeeSeiiiiS eZi``iiiiieiSeiiSOSie\eOe
5, pos: 69, distance: 1, reverse
@NB551106:9:H5Y5GBGX2:1:13201:25711:16931 1:N:0:GATCAG
GGTGCCGCCTGCTGGGCATCTGCCTCACCTCCCCCGTGC AGCTCATCATGCAGCTCATGCCCTTCGGCT C CCTCCTGGACTATGTCCGGGAACACAAAGA CAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGT
-
<//6E<AEE<A<E<A<A<E/E<A</E/EAEEE/EEEEAE /EEEAEEEAEEA/EE/EEEEEEEE/EEEEE E EEEEE/EEEEEEEEEEEAEEEEEEEEEEEE E6EE/EEEEEEEEEEEEEEEEEE6EEEEEEEEE/E/EEEEEEEEEEAAAA/
6, pos: 17, distance: 1, forward
@NB551106:9:H5Y5GBGX2:1:12311:3352:1406 2:N:0:GATCAG
GCTCATGCCCTTCGGCT C CCTCCTGGACTATGTCCAGGAACACAAAGA CAAAATAGGCTCCCAGAACCAGCTCAACTGGTGTGTGCAGCACGCAAACGTAATCAGGGAAGGCAGCTACGGGGAGGGGAGATAAGTCGCCAGGCGCCGCACC
+
AAAA/A/EEE/AEEEEE 6 EE/EE/EE/EEEA//EAE/E//E//AE/// EEE/E///6EE6EE///<EE/E/E/E/A<////EE/<6E///E/E<<E/E/<A/E<////A/EA/E///<A/EA///A///////////<//////A</EEA/
---------------
EGFR-pos-7-55242465:55242479-c.2235_2249del15-p.E746_A750delELREA-COSM6223 TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT chr7
1, pos: 170, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:22307:15360:1044 1:N:0:GATCAG merged offset:94 overlap:57 diff:0
CACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATCCACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCA
+
AAAAAEEEEEEEEEEEEEEAEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEE/EEAEEEEEEEEEEEAEEEA/E<EEEEAEEE/EEEEEEEAS`\`aO``SeeeS`J`\`SaeeaWJe=\ei`ieeieS```Z`ieiiie OeieeSeeSEEEEAEEEEEEEAEEEEEE EEAE AAEEEEEEEAEEEEEEEEEEEEEEEEEE EEEEEEEEEAEEEEEEEEEEEEEEEAEEEEEEEEEEEAAAAA
2, pos: 140, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:11309:4551:3202 1:N:0:GATCAG merged offset:63 overlap:87 diff:0
GCATCGCTGGTAACATCCACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCT
-
AAAAAEEEEEEEEEEEEEAEEEEEAEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEe`eZZSe`Seei`iiiieeeiei`eeea`iiieeiieii``iiiiiie ieSiiiiiiiiiiiiiiei`We`i`e`e iie` iiiieeZEEEEEEEEEEEEEEEEEEEEE EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA
3, pos: 125, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:21303:20073:9645 1:N:0:GATCAG merged offset:50 overlap:100 diff:0
TCCACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTG
-
AAAAAEEEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEZ`iSaeii`iie`eeiiiSeOSeeiSiiieiieeiiJiiiiiiiSee eiee`eiiSiieii`iii`Siiii`Sii iiii ieieaZeeeii`iiiiiiieiEEEEEEE EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE6EEEEEEAAAAA
4, pos: 123, distance: 2, forward
@NB551106:9:H5Y5GBGX2:1:21107:2005:9896 1:N:0:GATCAG
CACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCATCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTACGAAAGCCCACAAGGAA
+
A6AAAEAEEA/EEEE//AEEEE6EEE//E/EEAE/EEAE/AAE<//E/EEEE<E6E///<EEEAEEE//AA//EEAAEEEEE/A/E<A<EEEEEE //AEAEEEE//E</E/</E/EE/A//<A EEAE EA/A<//6AE/A6A/<<E/<//<
5, pos: 123, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:22310:18260:8674 1:N:0:GATCAG merged offset:40 overlap:110 diff:0
CACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCA
+
AAAAAEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEiiiiiiie`eieSiiiiiiieieiiiiiiieii`iiiiiiiiiieiiiiiiiiei iiiiiiiiii`iiieii``e\iieeeii iieS iie`eSeJiiiiieieiiiii`iEEEEE EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA
6, pos: 123, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:21306:3386:1754 1:N:0:GATCAG merged offset:40 overlap:111 diff:1
CACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCA
+
A/AAAEEAEAEEEEEEEAEEEEEEEEE6EE/EEEEEEE/E`\`OS`ZeZQa\SSeiiiiiiiiiiiei`SeeiiaSiii`iiie`O`iiiiS\ei eieiiSQSSiiieSii`ii`SSaae`Oa e`aJ S`ieiiiWiiSSei`eii`SiSZEEEEE EEEEEEEEEEEEEEEEEEEEAAEEEAEEEEAAAAA
7, pos: 123, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:12311:20781:15493 1:N:0:GATCAG merged offset:40 overlap:110 diff:0
CACCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCA
-
AAAAAEE6EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEESe`eeeeeZeie`e`Siiieeiieeeaiii`iiSeieieeeiiiSiSiieeiiie eiiiieiSieiiiiZieiieiiiiiaea `Sii `iSe`eiiieeeieSZe`eiee`EEEEE EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA
8, pos: 121, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:13212:8855:14707 1:N:0:GATCAG merged offset:23 overlap:128 diff:0
CCCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGC
-
AAAAAEEEEEE6EEEE6EEEEEEVai`e`SeieiieeiiieSiiiei\e`eiiiiiieiiiiiSiiiiieiiieieieiiiiSiieiea`iiS `iSW`SeSie`i`iiiiSi``ieiei`i iSiS iiiiiieeiiSaeei`iiSeSiZe``EE EEEEEEEEEEEEEEEEAAAAA
9, pos: 120, distance: 1, forward
@NB551106:9:H5Y5GBGX2:1:12107:16190:15233 2:N:0:GATCAG
CCAGATCACTGGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAACGCCAACAAGGAAATCC
+
6A6A/A//EAEEEE6EE//E6EEA/AE//EEA///6EEE///EAE//EEE//AA/</EE/6E6/6EAE/6/EAE///EEEEA/EA<EE/EEE AEEAAAEA<A/////<A//E/<EE//// /<// <E/E66/<A//<////A//EE/////6
10, pos: 110, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:13206:2898:10412 1:N:0:GATCAG merged offset:0 overlap:129 diff:0
GGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCA
-
\aaaae`iiiSeiiiiiieiiiiiiiiS`iiiiieei`iSiiieiiiiiiiiiiiiiiiiiiiiii`iiieiiiiieiiiee eiiiiiiiiiiiieeiiaiiieiiiiii eiie eiiiii`eiieO\ee
11, pos: 110, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:13108:15474:2849 1:N:0:GATCAG merged offset:21 overlap:128 diff:0
GGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGCGTGG
-
AAAAAEEEEEEEEEEEEEEEEieii`iSeeeiiieiiieiiiiiiiiiieiiieeiiii`ieeiiiiiiiZiiiieiiiiei iiiiieiiiiiieeii`iiiiiiiiiii iiii iiiiiieeiiS`eeii`iiiiiiiiiii iSiVeSaEEEEEEEEEEEEEEEEAAAAA
12, pos: 110, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:11210:20113:20326 1:N:0:GATCAG merged offset:21 overlap:128 diff:0
GGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGG
-
AAAAAAEEEEEEEEEEE/EEE`ieZeeei`eZiSiSJiiiiiii`ii`iiiS`Se`OeiiiiSieieeeZeieieiiiiSi` ii`iiSeiiSieOeiiJiZeeaieiiii `Sii iiiieee`ieS`e``e`iii`ei`eSe\ eSS``\ZEEEEEEEEEEEEEEEEAAAAA
13, pos: 110, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:12306:15839:8991 1:N:0:GATCAG merged offset:21 overlap:128 diff:0
GGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGG
-
AAAAAEEEEEEEEEEEEEEEEeeee``ieeeeiSe``eeiiiiiiiiieiiiiiiiiiiieiiiieeeiiiiiiiiiiiiii iiiiiiiiiiiiiiiieaeeSiiiiiii iiii iiiiieeiiiii`eiieiieeiiiieei `e`e`e`EEEEEEEEEEEEEEEEAAAAA
14, pos: 110, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:22106:2408:9991 1:N:0:GATCAG merged offset:21 overlap:128 diff:1
GGGCAGCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGG
-
AAAAAEEEEAEEEEEEAEEEEeieiEiSeieiiOSe`SeSi`iiJSeeiSiiiiiZ\e`iiieaii`i\ieiiieiiiiiii Siiieiii`iiieeii`ZeSeiiie`ei e`ee `J`eeSiiSS`SeiSe`ZSSeSZ``eOi ``\J\OZEEEEAEEEE6EEEEEEAAAAA
15, pos: 105, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:13106:22209:19593 1:N:0:GATCAG merged offset:0 overlap:133 diff:0
GCATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAA
-
a\a\eiieeii`SSiiiiiiSiiiSeiSiieiieSiiiiiiiiiei`eieiiiiieiiiieeiiiiiieiieSiiii ii`iiiiieiieiiieiiiieeaiii`i eiSi Si`iiiieiSiiieiiiiiaaV\e
16, pos: 103, distance: 1, reverse
@NB551106:9:H5Y5GBGX2:1:13304:5888:13157 1:N:0:GATCAG
ATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGGCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGC
-
<E/EEEEE6<</<E/<<<<AEAE<EEEE/EEEEEEA<EEEEAAAEAAEEEEE/<EEEEEAE<EEEEAEEEA<EEE EEEAEEEEEEEEEEEEAEEE/EEEEEEE EEEE EEEEEEAEEEEEEEEEEEEEEEEEEEEE EEEEEEEEEEEAAAAA
17, pos: 103, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:12111:18507:10428 1:N:0:GATCAG
ATGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGC
-
/EAE/A/AEE/A<</<<AAE/E<AA</E<EEE<EEEE/EEA/EAAAAEEAEEEA/EEAEAAEAEE/EEEEAEAEE AEEEEEEEEEEEE6EEEEEE/EEEE/AE EEAE EEEEEEEEEEAEEEEEEEEEEEEEEEEE EEEEEEEEEEE/AAAA
18, pos: 102, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:22110:25007:5842 1:N:0:GATCAG merged offset:0 overlap:134 diff:0
TGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT
+
\aO\V`Zeiiiei``iieiiiiiieiieii`SiiieiiSiiiiiiiiiiiiiii`iiiiiiiiiiiiiiiiiii iiiiiiiiiiiiiieiieeeiiiiiiii i`ei iiiiiieiiSii`ieiiieiiiSeeVO\
19, pos: 102, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:12305:9255:3059 1:N:0:GATCAG merged offset:0 overlap:139 diff:0
TGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATG
-
eaaaaiiieeiiiiieiiiieiiiiieiiiiiiiiiiiiiiiiiiieiiiiiiiiiiiiiiiiiiiiiiiiiii iiiiiiiiiiiiiiiiiieiiiiiiiei iiii iiiiieiiiiiiiiiiiiiii`iiiiei eeea\
20, pos: 102, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:13305:13527:11292 1:N:0:GATCAG merged offset:1 overlap:150 diff:0
TGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTT
+
AaeaOii``eieeee`SeiiiiiiiiiiSii`iiiiiiiiiiiiiiiiiiiiiiiiiieiieiiiieiiiie`i iiieeiiiiiiieiiiii`ieiiieiii ieii iiiieieiiiiiiiieiii`ieiiie`e `eiS`SSiiiiii\VaaA
21, pos: 102, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:22109:24401:1453 1:N:0:GATCAG merged offset:1 overlap:150 diff:0
TGTGGCACCATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCGGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTT
-
AVaVaSi`eiieeiiiiiii`Seieieeiii`eiiieiiiieiiiiiiiieiiiiieeiiiiiiiiiieeieii iiiiiSiiiiiiiiiiiiiiiieiiiei iiii iSieiee`iiiieeiiiiiieei`eeei `ee``eeiie`eiaaaaA
22, pos: 89, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:22103:7493:13522 1:N:0:GATCAG
CACATTTGAGAGTTAACGTCTTCCTTCTCTCTCTGTCTTAGGGACTCTTGATCCCAGAATG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGG
-
<//A/AEE///<EEEE/<//EA/EEE/E////AEEE6/E6/A/EEAAE/6<E/<<<A/A/E E6<EAA<<E<//EAEEEE/AEAA/EEE/ //// /EEEE/EEEEEE//AA/EA/EEA/E/EE /EEEEEAEE6EAEAEEEAEEEEEAAAAA
23, pos: 89, distance: 1, forward
@NB551106:9:H5Y5GBGX2:1:11207:12649:18883 2:N:0:GATCAG
CACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAACTCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGG
+
AAAAAEEEEEEEEE6/EEEEEAEEEEEEE6EEE6EEEEEEEEEEEEEEEAEAEEEEEEEEE EEEEAEEAE/EEEEEEEEEEEAEAEEEE EE/E <AEEAEEA<E//AAEEEEEE<EE/A/A< //<<<A/AA<//AA<A/AA/A//</A<A//
24, pos: 89, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:12202:5391:17111 2:N:0:GATCAG
CACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGG
+
AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEE EEEEEEEEEEEEEAEEEEEEEEEEEA/E EEE/ /EEAEAEE/E/AEEEEEEE/A/E<AAAE //<6///EA/<A/<<6/AA<EEAE/A/EEA
25, pos: 83, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:21107:2005:9896 2:N:0:GATCAG
TGCCAGTTAACGTCTTACTTCTCTCTCTGTCTTAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCA
-
<E///A/A//A<EAAE/EEEAEAE/EAAE<<</EE/////EEAEAE<E<<EEEEE E/EEAEEAEEEE<EE/AE/E/EEA<A/E AA/E AEEEEAEE<EAEAEE/EEEEEEE/EEEE /AEEEAEEEEAEAEAEEEEEEEEEEEEEEEA6AAA
26, pos: 82, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:11210:9514:13906 2:N:0:GATCAG
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATG
+
AAAAAE6/EEEEEEEEEEAEEEEEEEE6EEEAEEEEAEEEEEEEEEEEEEEAEE E6EAEEEEEEEEEEEEEEEAEAAEEAE6 /AE/ EAEE/6EAEEE/E//EA<AAE<A<E/AA E/<E</AE////A<A<<<A//A/A<A/<//////<A/
27, pos: 82, distance: 1, reverse
@NB551106:9:H5Y5GBGX2:1:11311:8139:8774 1:N:0:GATCAG merged offset:71 overlap:80 diff:1
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGATAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTCTGCTCTAGACCCTGCTCATCTCCACA
-
AA/AA/EA/A6/EE/EEEEAEEEEEEE/E/AEEEAEEEEEEEEE/EEE/EE/// /<EE/E/EEEEA/EEEESSSDOO`SESO iSae ie`iiieJ`S`eZ==SSiiOS`e`Sei` i`iSiOQ`SOSJeeVSi`aQeeiie\eJ`OSeia=e`AEEEEAE/AAEEEEEE/EEEEEE/EEEEEEEEE/EE//EAEA/6EEEEEEEEEEEEAEAAEEEEEEA6AAA
28, pos: 82, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:13306:19825:7070 1:N:0:GATCAG merged offset:71 overlap:80 diff:0
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTCTGCTCTAGACCCTGCTCATCTCCACA
-
AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE EEEEEEEEEEEEEAEEE`eZieee``e` eZSe eii`i`Z`Ze`iiiSe``Siie``iS`i Ze\\eS`iiSiiiS``\``e`ieii`eSiaeaiiieeEEAEEEEE<EEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA
29, pos: 79, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:21206:9257:20274 1:N:0:GATCAG merged offset:0 overlap:132 diff:0
AGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGC
-
eeaeO`iiSiSiieiiei`SeieieeieiQiZeeiiii`iSiieeeieSei iiSiSiZiiiZiiieeS`Qee`iiieie eeii `S``eSZe`eeiS`eeeeeeeSeSe`S` S`S`SeiS`SS`S`SSaO\\O
30, pos: 79, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:22302:9734:16623 2:N:0:GATCAG
AGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGC
-
A<A/AAA/EE/EE/E/EAEEEEEE</AEEAE//EAAE<<AEEA/EAEEA/E AAAAEAEEEEEEE//EEE<AA<6AEEEE EEEA 6E6AE/AEE/EEEEEEEEE/AEEEEEEE EEEA/EAEE6EEEEEEAAAA/
31, pos: 79, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:12101:16909:12188 2:N:0:GATCAG
AGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGC
-
EA<<<<EE/<<<AAE<AEAEAEEE<EEEEEEAEEEAEEAEEEAEEEEEEEE EEEEEE<<A/EEAEEAAEEEEEEE6EEE EA/E EEEEEEEEE6AAEEEEEEEEAEE/EEEE AEEEEEEEE/EEEEEEAAAAA
32, pos: 79, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:22302:9734:16623 1:N:0:GATCAG
AGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCAGATCGGAAGATCAAACGT
+
A/AAAEAEE/EEAEEEEEAEAEEAAAEAEEEEA6EEE/EAEE/EEEEEAEE EEAEEEEAEEEE6EEE/E/EEEA6AAEE EEEA AE/EE/<6AEEEAEEE<EEEAEEEE<6< 6<EE/</EEEEE6AA/EEEEAEAEEA<<E/AE/EE/E6<<
33, pos: 79, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:12101:16909:12188 1:N:0:GATCAG
AGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCAGATCGGAAGAGCTCACGG
+
A/AAAEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEE6EEEAEEEEEE EEEEEEEEEEEEEEEEAEEEE<AA6EEE AEEE EAEEAEEE//<EEAEEEAEEAA//EEAE EEEEEEE//EEEEE<AE<AEEEE/<<AAEE/E<A//<<A/
34, pos: 76, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:12204:16446:13427 1:N:0:GATCAG merged offset:0 overlap:127 diff:0
TAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTT
-
ea\eeiiiiiiiiiiiiiieiiiiiieiiiiiiiiieeiiiiiiiiii iiiiiiiiiiiiiiiiiiiiiiiiiiii iiii iiiiiiiiiiiiiiiiiiiiiii`ieei iieiiiieiZeiiiee\a\
35, pos: 76, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:12206:3492:9156 1:N:0:GATCAG
TAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTA
-
EEEEE/EEEEEEEEEEEE/EEEEEE<EAAEEEEEEEEEEEEEEEEEEE EEEEEEEEEEEEEEEEEEEEEEEEEEEE EEEE EEEEEEEEEEEEEEEEEEEEEEEEEEEE EEEEEEEEEEEEEEAAAAA
36, pos: 76, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:12206:3492:9156 2:N:0:GATCAG
TAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTT
+
AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE EEEEEEEEEE/EEEEEEEEEAEEEEEEE EEE6 EEEEEEEAEEEEEEEAEEEEEEEEEEEE EEAE<EEEEAEEE/AEEAE
37, pos: 76, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:21211:17320:5563 1:N:0:GATCAG merged offset:0 overlap:127 diff:0
TAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTT
-
aeaeeieeiiie`iieeiiiiiiiiiieiiieSiieeeiiieiiieii eie`iiiiiiiiiiii`iiieiiiiiii aiii iiiiiiieiiiiiiiiiiiiieii`iii iiiiiiiiiZ`ee`\aeae
38, pos: 76, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:22108:26312:10622 1:N:0:GATCAG merged offset:29 overlap:122 diff:0
TAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTG
-
AAAAAEEEEEEEEEEEEEEEEEEEEEEEEiieee`e``iee`Se`i`e ieie`Siiii`iS`iSiieeieeiieii ii`` iiSiiieiiSeieieSiieiiieeeiii iiia`iiiiiaeiieie`e`````ZSiieeeOiiiSSeieZieEEEEEEEEEEEEEEEEEEEEEEEEAAAAA
39, pos: 62, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:21206:25614:14503 1:N:0:GATCAG merged offset:0 overlap:117 diff:0
TCTCTCTGTCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTG
-
eeeeeiiiiiiiZi`iiiiiiiiiiiiiiiia`i iiiiiiiiiiiiiiiiiiSiiZ``iiie iiii ieiiieeiiiieeiiiiiiiiiiiiiii iiiiieiiiieiiiiiieeeeee
40, pos: 54, distance: 1, forward
@NB551106:9:H5Y5GBGX2:1:13202:3778:18913 1:N:0:GATCAG
TCATAGGGACTCTGGATCCCAGAAGG TGAGAAAGTTAAAATTACCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGTGGTCCATGGCTC
+
/AAAAEEE/EAEEAAEAEEE/AEE/E E6E6EEEEAEE/EEEE/EEAAAAA/EEE /EE/ E/EE//A/EEA/EEEEEEEE6A////<E E<<///</EE</EE/6<///A////AE/AE6<///E/AE/A
41, pos: 35, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:21108:9888:4646 1:N:0:GATCAG
CAGAAGG TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGAGAGTTTCTGCTTTGCTGTGTGTGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTC
+
AA6AA<6 /A/AEEEA6A/AAEEE/E//EEE/<A<E EAAE EEEE/EE/EEA/EAEAEEE6AEEE/6E/ E/EE//AE/EEEE/EE//EAEE/EE/A/EA/EEAAA//E/</E//AEEAAAE</A/E<E/AAEAAE//AA//AEAE</AEEA
42, pos: 28, distance: 1, forward
@NB551106:9:H5Y5GBGX2:1:22109:24061:12989 1:N:0:GATCAG
TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCAAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGAGTGGGGGTCCATGGCTCTGCACCTCAGGCCCA
+
AAAA<EEEE/AEEEEAEEAAE/EEE6EA AEEE EEEEEEE</EEEE/EEEEEEAEE/EA6E EAE/EEEEEEAE<A<AEAAEEEA//EA/AEE/A/AEE<<EEE//A<EEEEEE6A/6
43, pos: 28, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:13202:7218:7375 1:N:0:GATCAG merged offset:0 overlap:116 diff:0
TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCA
-
e=eae`iieiiieeSSieeSiiiiiiei e`i` iSJieaii`iSiiiiSiiiiSSiOiiii iSiiO=iiiiiZiieiiiiiVieiiiiiii`Siiiiaiieiii=ieiiieeeeSae
44, pos: 28, distance: 1, reverse
@NB551106:9:H5Y5GBGX2:1:22109:24061:12989 2:N:0:GATCAG
TGAGAAAGTTAAAATTCCCGTCGATATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCA
-
A<<6<<EEEEAEE/AE<//<EEE/EAAA <AEA EEE/EE/EE//EEE/EE/EEE///E/EE AA/AA/EEE/AAEEE/EEEEEEEEEEEEAEEEEEEEEA/AEEEEEEAEEEEAA//A
45, pos: 28, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:12312:12247:11795 1:N:0:GATCAG
TGAGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCAACCCTATTCTCATGACTGGCAGATTCTCTCCTCT
+
AA6A6EE6E/EE66EEE/AEEA/E/EA/ /E/E /E/EE//EE/////EEEE//EAAE/<EE E/E//E/E/A<E///EE/E</////E/E///EEA/EA//<6//EEAE/////////AA///EAA<<<<//</</EE/6/</<///A//
46, pos: 26, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:21205:17687:9844 1:N:0:GATCAG merged offset:35 overlap:116 diff:0
AGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTCTGCTCTAGACCCTGCTCATCTCCACATCCTAAATGTTCACTTTC
+
AAAAAEEEEEEEEEEEEEEEEEEEEE EEEE EEEEE`iieeeieeSi``iie`e`eeei eee`eiiiiiSSiiii`eiiiZeSeiSiiii`iiieii`SiiiSJ`iii`iii`iSiiS`ei`iie``OeiiiiiiiiiiJZeeS\e`SeSi`EEEEAEEEEEEEEEEEEEEEEEEEEEEEAAAAA
47, pos: 26, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:13302:10944:2114 1:N:0:GATCAG merged offset:35 overlap:116 diff:0
AGAAAGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTCTGCTCTAGACCCTGCTCATCTCCACATCCTAAATGTTCACTTTC
+
AAAAAEEEEEEEEEEEEEEEEEEEEE EEEE EEEEE`eeZ``e``Z`Z``eZZ`Sieei `ee`i`iieSiiaeiiiieiiiiiieiiiiieeiieiieeaaiSOei`ieiiii`Se`iee`eiSe`ieSiie`Sii`eee````SZZe`Z`ZEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAAAA
48, pos: 22, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:12211:24962:16940 1:N:0:GATCAG merged offset:31 overlap:120 diff:1
AGTTAAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTCTGCTCTAGACCCTGCTCATCTCCACATCCTAAATGTTCACTTTCTA
+
AAAAAEEEEEEEEEEEEEEEEE EEEE EEEEEiSieSSieeSSZS`eeeSSeSe\ ieeeiea``ZSE`SieOiiiiii`iSeiieeeeiieiiiOiaiSaiiieaeiiOiSSieeeeeee`iSeeieiiSe`ee`````e=ieeSiiSieSSEEEEEEEEEEEEEEEEEEE6EAEEEEAAAAA
49, pos: 18, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:21111:2651:10876 1:N:0:GATCAG
AAAATTCCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTCTGCTCTCGACGCTGCTCAT
+
AAAAAEEEAEEEEEEEEE EEEE EEEEEEEEEEAEEEEEEEEEEEEEEEEE EEEEEEEEEE/EEEEEEEEEEAEEEEAEEEEEE6EAAEEEEEE//E/EE/E/E6A/A<EAEEEEEEEAE</</A/<<AE/6EE/A<<A///</</6AAA<<
50, pos: 12, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:22308:19431:1802 1:N:0:GATCAG merged offset:0 overlap:131 diff:1
CCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTCTGCTC
+
\aeOaeiaeSi` `eS` ``Seiei\iJEeiiiSeSSSieSi`iii ieSSeSeiaSSiiiiiSiiieei=iOeeiiVeieSieeiieie`eieiieiiOi`eiOiSieOeeiiSS`iieaSiiii`OZeaa\\
51, pos: 12, distance: 0, forward
@NB551106:9:H5Y5GBGX2:1:12201:2567:10967 1:N:0:GATCAG
CCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCCGCTGCTCTGCTC
+
AAAAAEEEEEEE EEEE EEEEEEEEAEEEEEEEEEEEEEEEEEEE EEEEEE6EEAEEEEEEAEEEE/EE/AEEAE/EEAEE/AEEA/EEAAEEEE/EEEE/EEA/6EEEEEAEEAEEEE//AEEEEE/EEAA
52, pos: 12, distance: 0, reverse
@NB551106:9:H5Y5GBGX2:1:12201:2567:10967 2:N:0:GATCAG
CCCGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGAAATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTCTGCTC
-
/A/E/<E<A<AA EE/E E/EE/AEAEE<EEE</EAEEEE</<E/E EEEEE6AEE/</EEEEEAEEA/EEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE6EEEEEEE6EEE/EAEEE/EEEEEEAAAAAA
53, pos: 12, distance: 2, reverse
@NB551106:9:H5Y5GBGX2:1:21108:9888:4646 2:N:0:GATCAG
CACGTCGCTATC AAAA CATCTCCGAAAGCCAACAAGGATATCCT CGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGCTCTGCTCTAGACCCTGCTCATCTCCAC
-
</<6</<//AE/ <A// /<EAEE/<E/EE<A/E///////EEEEE EE<////<E/E/E/EE<E/<EA6//EE/E/</E</E<EEEEA/A/EEEEAE/EEEE66EEEEEAEEEEE//E/AE<EEE6EEE//EEEEEE/EEEA6EEEEEAA6A/
./mutscan -1 R1.fq.gz -2 R2.fq.gz
Mutscan v1.14.0, time used: 28 seconds
from mutscan.
I am just running it on the files in the testdata
directory, R1.fq.gz and R2.fq.gz, which are indeed small. However, I also see the same output on some of my own data, which runs for 49 seconds. I am sure the mutations are there because I can find them with fgrep
.
from mutscan.
What are the mutations in your own data?
Probably it's not included in the built-in targets.
from mutscan.
I was providing my own mutation, but I just realized my definition for R
was wrong. It seems to be working now, thanks. I would suggest fixing the data in the testdata
folder to output something as well though, as this suggested to me the problem was not on my end.
from mutscan.
Related Issues (15)
- TODO: generate HTML report HOT 1
- Feature request: add txt output and coverage information to the mutations HOT 2
- Retrieve readids supporting mutations.
- the latest mutscan cannot be work with VEP-annoated vcf ?
- Deadlock with the --thread option
- How to use `mutscan` with UMI ? HOT 1
- Missing reads with `--simplified on`
- How to make VCF-format mutation file ?
- Build error with g++ 6.6.1 on RHEL 6.5 HOT 1
- compile error HOT 7
- About VCF format HOT 2
- HTML report can't work while copy it to windows, and may be a output directory needed. HOT 12
- Manuscript/Preprint? HOT 2
- can not find/display variants which observed in IGV HOT 2
Recommend Projects
-
React
A declarative, efficient, and flexible JavaScript library for building user interfaces.
-
Vue.js
🖖 Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.
-
Typescript
TypeScript is a superset of JavaScript that compiles to clean JavaScript output.
-
TensorFlow
An Open Source Machine Learning Framework for Everyone
-
Django
The Web framework for perfectionists with deadlines.
-
Laravel
A PHP framework for web artisans
-
D3
Bring data to life with SVG, Canvas and HTML. 📊📈🎉
-
Recommend Topics
-
javascript
JavaScript (JS) is a lightweight interpreted programming language with first-class functions.
-
web
Some thing interesting about web. New door for the world.
-
server
A server is a program made to process requests and deliver data to clients.
-
Machine learning
Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.
-
Visualization
Some thing interesting about visualization, use data art
-
Game
Some thing interesting about game, make everyone happy.
Recommend Org
-
Facebook
We are working to build community through open source technology. NB: members must have two-factor auth.
-
Microsoft
Open source projects and samples from Microsoft.
-
Google
Google ❤️ Open Source for everyone.
-
Alibaba
Alibaba Open Source for everyone
-
D3
Data-Driven Documents codes.
-
Tencent
China tencent open source team.
from mutscan.