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View Code? Open in Web Editor NEWA simplified pipeline for ctDNA sequencing data analysis
License: MIT License
A simplified pipeline for ctDNA sequencing data analysis
License: MIT License
https://github.com/OpenGene/ctdna-pipeline/blob/master/pipeline.sh#L24 says:
java -jar VarScan.v2.3.8.jar mpileup2snp outdir/test.dedup.mpileup --min-coverage 4 --min-reads2 2 --min-avg-qual 20 --min-var-freq 0.001 --min-freq-for-hom 90 --output-vcf 1 >outdir/test.snp.vcf
Now I'm not sure whether the value of --min-freq-for-hom 90
is OK. Because now the new version of VarScan (v2.4.3) says:
USAGE: java -jar VarScan.jar mpileup2cns [pileup file] OPTIONS
mpileup file - The SAMtools mpileup file
OPTIONS:
...
--min-freq-for-hom Minimum frequency to call homozygote [0.75]
...
Note, the default value is 0.75 rather than 75 (%). Therefore perhaps we should use value 0.9 rather than 90 (5)
Is there any update to the pipeline?
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