Comments (5)
We could consider doing this, it'll be a bit hairy since it's using multiple processes
from duplex-tools.
How you would use the output though? It can often be a pain to parse alignment that's dumped as ascii. Perhaps it would be better to have a table of matches? Would that be better/useful?
read_id | start | end |
---|---|---|
befd-... | 20 | 30 |
befd-... | 120 | 130 |
from duplex-tools.
It is useful to have an entire record of where and how close the alignments are for all hits as opposed to the first hit in the case of multiple splits.
At least the position, start, and end would be good to have for each alignment that meets the edit distance threshold for each read. Can then extract these sequences myself from the original input read to look at the alignment.
from duplex-tools.
Makes sense, I've made a MR internally that should be looked through quite soon
from duplex-tools.
Makes sense, I've made a MR internally that should be looked through quite soon
Thanks!
from duplex-tools.
Related Issues (20)
- Unexpected input file name changes output file file name format on split_on_adapter HOT 1
- problem with Fastx HOT 2
- Cannot install duplex_tools HOT 2
- split_pairs not working HOT 3
- pairs.txt file empty, but pairs_from_bam/pair_ids.txt not empty HOT 1
- empty output from split_pairs HOT 5
- KeyError: 'sequence_length_template' when basecalling is turned off HOT 2
- Duplicate reads and read splitting option in MinKNOW HOT 9
- Positional arguments (especially seqkit_stats_nosecondary) in duplex_tools assess_split_on_adapter HOT 1
- split_pod5 supported seed types error HOT 3
- pod5 version of duplex_tools issue HOT 3
- question on split pairs HOT 3
- promethion good pairs: 0 HOT 3
- Extracting duplex reads for multiplexed samples HOT 2
- Unexpected base in duplex call HOT 4
- issue with split_on_adapter output HOT 17
- couldn't install on linux or pc HOT 5
- np.bool deprecated, package no longer works HOT 1
- split_on_adapter no more than one core?
- guppy_duplex ValueError: not enough values to unpack (expected 4, got 3) HOT 5
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