Comments (1)
- not available on conda
- python 2.7 --> outdated
- will wait until it is included in the crisprScore package, where a request was already made
from snakemake-crispr-guides.
Related Issues (20)
- Add functionality to deal with guides targeting multiple loci (e.g. duplicated) genes
- Feature requests: add function to design random control guide RNAs HOT 1
- improve flexibility of handling `gff` input files
- add feature to export guide list as GFF file for visualization
- When using assembly: "GCF_005577135.1", species name is not parsed correctly, which is causing failure in create_bsgenome.R and design_guides.R HOT 4
- add possibility to create guides by tiling only, or by tiling for intergenic regions (mixed) HOT 1
- Additional types of CDS annotations HOT 2
- workflow is listed in snakemake wf catalog, but not as "standardized usage" HOT 3
- feature request: option to add additional space around CDS when designing intergenic guides
- improve output figures for intergenic regions when using tiling approach
- Feature request: add score based on distance to TSS
- change license to MIT
- minor issues with WD when sourcing script, and ggplot2
- Add module to generate BSgenome --> enables bowtie as default off-target search HOT 1
- Feature request: add score based on G content of seed region
- Testing fails on GH actions with snakemake test workflow
- Test behavior with alternative options HOT 1
- add overview figures for pipeline and guide RNA localization on strands
- avoid overlap between TSS window (5' UTR) and 3' terminus of upstream gene
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from snakemake-crispr-guides.