Comments (4)
When checking, it also did not become obvious to me if the downloaded meta data is anyways of importance for downstream analyses etc.
If it is not, a possible solution might be to just simply give some mock species ID or species name and ignore the meta data.
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Checked again and "Picosynechococcus sp. PCC 11901" does not seem to be a valid species name either, so "Synechococcus sp. PCC 11901" would have to be entered instead, which is not part of the fasta headers or given in the .gff file which were downloaded. A possible solution would be to extract the species field given in the gff file - even though I am not sure if all gffs contain this field (in case of Synechococcus 11901 the following line: ##species https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2579791
)
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good find!
will try to reproduce this and see how to fix. Ideally there is an automatic solution such that the user does not need to specify the species name manually
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@ute-hoffmann check out dev
branch, where this is fixed now.
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Related Issues (19)
- Add functionality to deal with guides targeting multiple loci (e.g. duplicated) genes
- Feature requests: add function to design random control guide RNAs HOT 1
- improve flexibility of handling `gff` input files
- add feature to export guide list as GFF file for visualization
- Feature request: add CRISPRoff score HOT 1
- add possibility to create guides by tiling only, or by tiling for intergenic regions (mixed) HOT 1
- Additional types of CDS annotations HOT 2
- workflow is listed in snakemake wf catalog, but not as "standardized usage" HOT 3
- feature request: option to add additional space around CDS when designing intergenic guides
- improve output figures for intergenic regions when using tiling approach
- Feature request: add score based on distance to TSS
- change license to MIT
- Add module to generate BSgenome --> enables bowtie as default off-target search HOT 1
- Feature request: add score based on G content of seed region
- Testing fails on GH actions with snakemake test workflow
- Test behavior with alternative options HOT 1
- add overview figures for pipeline and guide RNA localization on strands
- avoid overlap between TSS window (5' UTR) and 3' terminus of upstream gene
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