Comments (3)
Hi @orangelc1221,
the error that you are seeing is one of the reasons why we recommend to use mamba. The picard environment somehow seems to be causing trouble for conda, no idea why, and it works fine with mamba. Hence, I recommend that you use conda to install mamba :-) You can for example add it to the grenepipe
environment itself, that you already created. That way, you can use mamba even on a cluster that does not yet have it.
As for your second issue with the cluster script run.slurm
: I have no idea what the rest of these guidelines say, or what that script contains, so I cannot really comment on that without further knowledge of the situation. However, we do provide our own guide for running snakemake and grenepipe in cluster environments. In particular relevant seems to be the part on what you described as "just run on the terminal": That usually won't work, as the snakemake command will then inevitably stop once you want to close that terminal. At the least, you will have to run snakemake from within screen
or tmux
, see above guide.
Hope that helps, so long
Lucas
from grenepipe.
Thank you!
from grenepipe.
You're welcome! For future reference: Did this solve all issues you had, and is it working now?
from grenepipe.
Related Issues (20)
- bwa-mem2 "{tmp}.0000.bam": File exists HOT 5
- threads for bwa-mem2 via slurm HOT 2
- Error running toy example HOT 6
- MissingRuleException HOT 13
- PID error HOT 9
- java.lang.OutOfMemoryError: Java heap space HOT 2
- GRENEPIPE v12.1 HOT 5
- Make "trimming-tool" optional HOT 4
- restrict-regions and short contigs HOT 2
- ModuleNotFoundError: No module named 'chardet' HOT 2
- Write full executed command for each step to log files for reproducibility HOT 3
- merging calls from multiple pipeline runs? HOT 2
- mamba is difficult to install in grenepipe environment HOT 6
- Feature Request: Download reference genome and known variation HOT 2
- config file HOT 5
- greenepipe run error HOT 5
- problem with dedup HOT 4
- a new type of error HOT 2
- a new type of error HOT 1
- another type of error HOT 11
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from grenepipe.