This is a software package for protein engineers. It uses protein structure and sequence information to aid researchers in designing protein recombination libraries.

These tools can calculate SCHEMA energies of chimeric proteins and run the RASPP algorithm to find optimal library designs. The package includes documentation and examples.

SCHEMA was developed in the laboratory of Frances H. Arnold at the California Institute of Technology.

References:

Voigt, C. et al., "Protein building blocks preserved by recombination," Nature Structural Biology 9(7):553-558 (2002). Meyer, M. et al., "Library analysis of SCHEMA-guided recombination," Protein Science 12:1686-1693 (2003). Otey, C. et al., "Functional evolution and structural conservation in chimeric cytochromes P450: Calibrating a structure-guided approach," Chemistry & Biology 11:1-20 (2004) Silberg, J. et al., "SCHEMA-guided protein recombination," Methods in Enzymology 388:35-42 (2004). Endelman, J. et al., "Site-directed protein recombination as a shortest-path problem," Protein Engineering, Design & Selection 17(7):589-594 (2005).