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jiangzy26 avatar jiangzy26 commented on August 27, 2024

I downloaded the re-sequencing data from NCBI, it is only one species, why there are FivePCGs of other species? what are the implications? The best close species of these five contigs is not the species itself, I am not sure whether there are problems, could you share your help? Thank you very much

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linzhi2013 avatar linzhi2013 commented on August 27, 2024

In the MitoZ paper,

In addition, Sequences, regardless of gene conflicts, containing ≥ 5 PCGs (complete or not) will be retained to confirm identities, e.g. parasites.

Therefore, the users should further check what the "_FivePCGs" sequences belong to. The first and easy step, is to do a NT blast (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome)

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linzhi2013 avatar linzhi2013 commented on August 27, 2024

See also https://github.com/linzhi2013/MitoZ/wiki/Tutorial#7-what-now

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jiangzy26 avatar jiangzy26 commented on August 27, 2024

Thank you very much for your kind reply, I'll read these carefully.

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