Comments (4)
I downloaded the re-sequencing data from NCBI, it is only one species, why there are FivePCGs of other species? what are the implications? The best close species of these five contigs is not the species itself, I am not sure whether there are problems, could you share your help? Thank you very much
from mitoz.
In the MitoZ paper,
In addition, Sequences, regardless of gene conflicts, containing ≥ 5 PCGs (complete or not) will be retained to confirm identities, e.g. parasites.
Therefore, the users should further check what the "_FivePCGs" sequences belong to. The first and easy step, is to do a NT blast (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome)
from mitoz.
See also https://github.com/linzhi2013/MitoZ/wiki/Tutorial#7-what-now
from mitoz.
Thank you very much for your kind reply, I'll read these carefully.
from mitoz.
Related Issues (20)
- Issue with tRNA annotation HOT 2
- Error in subcommand annoate in the Singularity image MitoZv3.4. HOT 2
- Problem with annotation HOT 1
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- overlap is 0 HOT 9
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- Missing tRNA annotation HOT 8
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from mitoz.