Comments (3)
Thanks for the report! I agree that this is strange behavior and I will add functionality to handle it in the next update.
Originally, I thought of slingshot()
as being the main function and getLineages
and getCurves
as being low-level helpers that most people wouldn't interact with, so I didn't think it was necessary to add a method for this case. But it seems like a few people have had this issue, especially with larger datasets where getCurves
can be slow.
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Oh, sorry, I also should have mentioned that you can work around this by extracting the relevant parts of the SingleCellExperiment
object and directly inputting them to getLineages
. In your case, I think that would look like this:
getLineages(reducedDims(spangler.sce)$PCA, clusterLabels = cl)
Which will return a SlingshotDataSet
object that can be used with getCurves
.
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Sorry for the delay. This is now implemented in the master branch here and in the devel branch of Bioconductor (slingshot version >= 1.3.1). Specifically, getLineages()
and getCurves()
now accept SingleCellExperiment
objects as input. Thanks for the report!
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Related Issues (20)
- trajectory bootstrapping and uncertainty HOT 3
- Slingshot pseudotime with CellRank HOT 8
- error when trying to overlay the lineage trajectory on umap HOT 2
- Slingshot curves look weird HOT 2
- Trajectory over condtions not ending in center HOT 1
- steps you have to take after slingshot HOT 1
- one question about psuedo time inference HOT 1
- pseudotime distribution is uneven when run slingshot on a subset of dimensionality reduction HOT 6
- What assay to use after Seurat integration HOT 2
- Error plotting the trajectory HOT 2
- Clustering discrepancy with Slingshot and Seurat HOT 5
- Error with SlingshotDataSet(): as(<dsCMatrix>, "dgCMatrix") is deprecated HOT 5
- Porting slingshot to Python HOT 4
- Calculating cell weights HOT 4
- Scale or not to scale the genes by their variance in real datasets HOT 2
- the cluster in end.clust is not the end of pseudotime value HOT 4
- Running Multiple Times Results In Some Cells Not Plotting HOT 3
- How to plot the pseudotime for all lineages together in one UMAP plot? HOT 3
- Error running slingshot: system is computationally singular HOT 2
- Calculating on PCs + Plotting On UMAP HOT 4
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