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nexflow_pipelines

Requirements:

  • nextflow (19.04)
  • JAVA >= 1.8 (Add this to your .bashrc: export NXF_JAVA_HOME=/etc/alternatives/java_sdk_1.8.0/)
  • For demultiplexing from S3: setup S3 credentials (the configuration for gis-nanopore-archive has to be your default profile, see here

To run the qcat pipeline:

nextflow qcat.nf --path s3://gis-nanopore-archive/GRIDION/N253_FLO-MIN106_SQK-LSK109/20190709_0628_GA30000_FAK66486_eb66a08d/fastq_pass/

porechop is officially retired

To run the pipeline:

nextflow  porechop.nf --path /mnt/seq/gridion/N101_FLO-MIN106_SQK-LSK108/ -resume

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