- nextflow (19.04)
- JAVA >= 1.8 (Add this to your .bashrc:
export NXF_JAVA_HOME=/etc/alternatives/java_sdk_1.8.0/
) - For demultiplexing from S3: setup S3 credentials (the configuration for gis-nanopore-archive has to be your default profile, see here
To run the qcat pipeline:
nextflow qcat.nf --path s3://gis-nanopore-archive/GRIDION/N253_FLO-MIN106_SQK-LSK109/20190709_0628_GA30000_FAK66486_eb66a08d/fastq_pass/
To run the pipeline:
nextflow porechop.nf --path /mnt/seq/gridion/N101_FLO-MIN106_SQK-LSK108/ -resume