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DNA methylation is a major epigenetic modification regulating several biological processes. A standard approach in the study of DNA methylation is bisulfite sequencing (BS-Seq). BS-Seq couples bisulfite conversion of DNA with next generation sequencing to provide a genome wide profile of DNA methylation at single base resolution. The analysis of BS-Seq data involves the use of customized aligners for mapping reads and additional bioinformatic pipelines for downstream data analysis. While most post-alignment programs generate methylation calls, MethGo carries out subsequent genomic and epigenomic analyses to comprehensively explore BS-Seq datasets.

MethGo is a simple and effective tool designed for the analysis of data from whole genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS). MethGo provides 5 major modules:

• COV: Coverage distribution of each cytosine
• MET: Both global and gene-centric cytosince methylation levels
• TXN: Cytosine methylation levels at transcription factor binding sites (TFBSs)
• SNP: Single nucleotide polymorphism (SNP) calling
• CNV: Copy number variation calling

MethGo is available under the terms of the MIT license.

• Documentation
• Source code
• Project page