This is a collection of scripts, which can mainly be used to export time-tag time-resolved fluorescence spectroscopic data collected on single-molecule or FCS setups.

This library of scripts is based on tttrlib.

Scripts are prepared for up to four different channels (green-parallel, green-perpendicular, red-parallel, and red-perpendicular). Colors are named according to the standard FRET-experiments with a "green" donor and a "red" acceptor fluorophore. For a four channel setup, data can also have been collected in the Pulsed Interleaved Excitation (PIE) mode and be exported based on "prompt" and "delay" time windows. Here a 50:50 split of the total time window is assumed.

Scripts labeled "_joined" append all data sets in the specified folder with a certain search term (e.g. A488*.ptu) and calculate either the sum (for decay histograms) or mean and standard deviation (for correlation analysis).

Correlation scripts labeled with "_stdev" split the data set to be correlated in three pieces, correlate each separately and report mean and standard deviation for this data set from these pieces.

For batch export, use scripts in the "batch" folder and modify the respective settings file. Usage of batch export: .....python batch_scripts.py --settings settings.yaml --path "path-to-data"