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sjessa avatar sjessa commented on July 28, 2024 1

Hi Yile,

The integration script for harmony is at code/scripts/integrate_harmony.R.
I've added some more details in this section: https://github.com/fungenomics/HGG-oncohistones/blob/master/README.md#data-integration

Selin

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sjessa avatar sjessa commented on July 28, 2024

Hi, thanks for the kind words and for checking out the code!

Thanks for catching that. The first file you mentioned was located outside this directory, but I have added a copy to this repo. You can now find the folder which is referenced as SEURAT_V3_ASSETS or file.path(params$assets, "resources") in the various preprocessing_*.Rmd scripts here: https://github.com/fungenomics/HGG-oncohistones/tree/master/code/seurat_v3_resources, and that contains CellCycleGeneList_1134_whitfield_2002_mice_gene_symbols.txt.

For the second, the script to produce that file is here: https://github.com/fungenomics/HGG-oncohistones/blob/master/code/scripts/make_scATAC_refs.R. It essentially follows directions in the Signac package.

Selin

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yilevine avatar yilevine commented on July 28, 2024

Hi Selin,

Really thanks for the reply and for sharing the code and the data.
I also would like to know how you integrated multiuome data (RNA + ATAC) across samples. I didn't find the related codes.
Could you also share it?
If I missed it, could you tell me where I can find it in this repo?
Thanks!

Best,
Yile

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yilevine avatar yilevine commented on July 28, 2024

Hi Selin,

Thanks a lot! I will have a look.

Yile

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