frietzelabuvm / ssvqc Goto Github PK
View Code? Open in Web Editor NEWR package for QC of enrichment based NGS assays. ChIP-seq, cut&run, ATAC-seq, etc.
License: Other
R package for QC of enrichment based NGS assays. ChIP-seq, cut&run, ATAC-seq, etc.
License: Other
Hi -
Does "0" on the x-axis of the signal heatmaps correspond to peak summit or peak center (or TSS)?
Thanks!
Hi,
do you have config files templates and/or a format description ?
Thanks
Hi,
How do I set up read_mode to bam_PE please?
Cheers!
Hi,
I am new using the tool and I was following the description on the main page using
path <- "/ATAC-PBAF/AK62/Data/QC"
peaks_files <- dir(path, pattern = ".broadPeak", full.names = TRUE)
bw_files <- dir(path, pattern = ".bw$", full.names = TRUE)
Then when I try to run
# Create ssvQC object
options(mc.cores = 4)
sqc = ssvQC(np_files, bw_files)
sqc = ssvQC.runAll(sqc)
I got the following message
Error in names(cols) <- group_names :
'names' attribute [15] must be the same length as the vector [8]
More info about the input files
head (broakPeak)
3067110 3067542 AK62_Ar_1_REP1.mLb.clN_peak_1 63 . 4.78408 8.36646 6.38689
1 3183757 3184089 AK62_Ar_1_REP1.mLb.clN_peak_2 35 . 3.67095 5.42779 3.56175
> length(peaks_files)
[1] 15
> length(bw_files)
[1] 15
Any advise?
Thanks!
Hi @ssvQC,
I am trying to run ssvQC on my Cut&Run files and I am getting this error:
Error in quantile.default(frip_dt$frip, c(0.1, 0.96)) :
missing values and NaN's not allowed if 'na.rm' is FALSE
Is there a way to set na.rm=TRUE while running the package?
Thank you
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