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This is the repository for the manuscript “Expanding the transcriptomic toolbox in prokaryotes by Nanopore sequencing of RNA and cDNA molecules.” In this study, we applied and benchmarked all currently available RNA-seq kits from Oxford Nanopore technologies to analyse RNAs in the prokaryotic model organism Escherichia coli K-12.

Home Page: https://felixgrunberger.github.io/microbepore

License: MIT License

R 100.00%
nanopore-sequencing

microbepore's Introduction

  • 👋 Hi, I’m @felixgrunberger
  • 👀 I’m interested in Microbiology 🦠, Transcriptomics, Sequencing, Data analysis 💻, Data visualization 📊 (in R) and many more things
  • 🌱 I’m currently learning how to use Nanopore RNA-seq data to expand the transcriptomic toolbox in prokaryotes ✌️
  • 📫 Contact me on twitter

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microbepore's Issues

ModuleNotFoundError: No module named 'idr' : termseq-peaks

Hi Felix,
Got an error while ran the script for calling the peaks.

$ termseq_peaks /media/majorram/Analysis_Data/singhrn/transcriptome_atlas/tss/trimmed/output/barcode07.plus.bedgraph /media/majorram/Analysis_Data/singhrn/transcriptome_atlas/tss/trimmed/output/barcode08.plus.bedgraph /media/majorram/Analysis_Data/singhrn/transcriptome_atlas/tss/trimmed/output/barcode09.plus.bedgraph --peaks /media/majorram/Analysis_Data/singhrn/transcriptome_atlas/tss/trimmed/output/barcode789.plus.peaks --strand +
Traceback (most recent call last):
File "/home/majorram/anaconda3/bin/termseq_peaks", line 11, in
from peaklib.peaklib import idr_peak_calls
File "/home/majorram/anaconda3/lib/python3.9/site-packages/peaklib/peaklib.py", line 6, in
from idr import idr
ModuleNotFoundError: No module named 'idr'

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