elizhughes Goto Github PK
Name: Liz Hughes
Type: User
Twitter: lizhughes21
Name: Liz Hughes
Type: User
Twitter: lizhughes21
To check the phenotype of Agrobacterium mutant double GAT201 strains in RPMI at 37 degrees
This code will produce a figure that demonstrates that we can successfully transform and do complementation in Cryptococcus.
Supplementary data for Gat201 paper, May 2023.
Cryptococcus neoformans RNA-seq Gat201 strains 2021.
Wallace Lab website
Cfu assay for WT, Gat201 deletion, Complemented clones C26 and C30
RTqPCR of WT, Gat201 deletion, Complemented clones C26 and C30. Genes tested Gat201, Gat204 and Liv3. 3 reference genes.
Analysis of homology of Cryptococcus neoformans Gat201 and related GATA-family transcription factors
Repo to learn git
Notes on how to use Git and GitHub.
RPMI vs CO2 independent media, KN99-alpha and GAT201 deletion mutant, 37 degrees
To check the phenotype of downstream targets of GAT201 in RPMI at 37 degrees
Set up to test growth of WT, Gat201 Madhani deletion and Gat201 complemented strains (C26 and C30) in YPD (NA) and YPD + 20mM HEPES at pH= 4 (4), pH=5.5 (5.5), pH = 7 (7) and pH = 8.5 (8.5) at 37 degrees.
Template repository for QuantSeq Fwd analysis, for RNA-seq data
Test growth of WT H99 and Gat 201 deletion mutants 003 (Madhani) and 004 (Bahn) in RPMI and YPD at 37 degrees.
This repo analyses RTQPCR data for Laura Tuck's Crypto Mobius paper. It will show the successful complementation of the GAT201 gene into the Madhani deletion mutant using the Crypto Mobius method.
qPCR analysis with the tidyverse
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