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atmutscreeen_gat201

atmutscreen

To check the phenotype of Agrobacterium mutant double GAT201 strains in RPMI at 37 degrees

cryptofig_b2

This code will produce a figure that demonstrates that we can successfully transform and do complementation in Cryptococcus.

cryptogat201_2023_suppdata

Supplementary data for Gat201 paper, May 2023.

cryptogat201rnaseq_draft

Cryptococcus neoformans RNA-seq Gat201 strains 2021.

djtf

ewallace.github.io

Wallace Lab website

gat201_cfu_assay

Cfu assay for WT, Gat201 deletion, Complemented clones C26 and C30

gat201_rtqpcr_assay

RTqPCR of WT, Gat201 deletion, Complemented clones C26 and C30. Genes tested Gat201, Gat204 and Liv3. 3 reference genes.

gat201homology_2022

Analysis of homology of Cryptococcus neoformans Gat201 and related GATA-family transcription factors

learn-git

Repo to learn git

learn_git_eh

Notes on how to use Git and GitHub.

planets

pr27_rpmivsco2indep

RPMI vs CO2 independent media, KN99-alpha and GAT201 deletion mutant, 37 degrees

pr_data_gat201

pr_gat201targets

To check the phenotype of downstream targets of GAT201 in RPMI at 37 degrees

pr_ypd_ph

Set up to test growth of WT, Gat201 Madhani deletion and Gat201 complemented strains (C26 and C30) in YPD (NA) and YPD + 20mM HEPES at pH= 4 (4), pH=5.5 (5.5), pH = 7 (7) and pH = 8.5 (8.5) at 37 degrees.

quantseqfwd_template

Template repository for QuantSeq Fwd analysis, for RNA-seq data

rpmi_ypd_prassay

Test growth of WT H99 and Gat 201 deletion mutants 003 (Madhani) and 004 (Bahn) in RPMI and YPD at 37 degrees.

rtqpcr_cryptomobiuspaper

This repo analyses RTQPCR data for Laura Tuck's Crypto Mobius paper. It will show the successful complementation of the GAT201 gene into the Madhani deletion mutant using the Crypto Mobius method.

tidyqpcr

qPCR analysis with the tidyverse