Comments (4)
pabloacera
commented on June 6, 2024
1
To our understanding, the RNA base calling model hasn't been updated since version 3 up to 6.1.2, so running guppy with default parameters past version ~3 doesn't affect base calling quality.
Cheers.
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EduEyras
commented on June 6, 2024
It should also work with Guppy 5 and later versions
In the paper we used Guppy 5 for the cell line data.
The github indicates Guppy 4.0.11+ meaning it should that version or later.
cheers
Eduardo
…On Fri, 9 Sept 2022 at 00:43, Lolita Lecompte ***@***.***> wrote:
Hello,
Thank you for developping CHEUI.
I was wondering if the reads must be basecalled with Guppy v4.0.14?
Can I use a later version of Guppy (in my case v6.0.1)? Will it impact the
performance of CHEUI?
Best,
Lolita
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callumparr
commented on June 6, 2024
I have all my data basecalled with guppy 3.4.5 would it be OK to use this version? as stated above I believe the RNA002 hac model hasn't changed since v3, basically when RNA got a hac model.
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EduEyras
commented on June 6, 2024
Hi Callum
Yes, it will be fine.
Best
E
On Thu, 13 Oct 2022 at 02:20, callumparr ***@***.***> wrote:
I have all my data basecalled with guppy 3.4.5 would it be OK to use this
version? as stated above I believe the RNA002 hac model hasn't changed
since v3, basically when RNA got a hac model.
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