Comments (1)
Hi,
Yes, the second column in the read_level_prediction_m6A_sorted will provide the read-level prediction probability from model 1. Then we have model 2 that takes those probabilities and predicts site-level probabilities. The site-level predictions provide the stochiometry that is calculated from the read-level probabilities of model 1. We used a double cutoff of 0.7 and 0.3 for read-level predictions. So you can consider reads with probability >= 0.7 to be methylated and probability <=0.3 non-methylated.
I hope it helps!
Thanks,
Akanksha
from cheui.
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