Comments (8)
Numpy recommendation downgraded to 1.19.5. Thanks Rania!
from cheui.
Hi,
it should be fine if the TensorFlow is installed properly.
Best
E.
from cheui.
Hi @EduEyras
Thanks for your fast reply.
I have another question, I would like to test CHEUI and other tools on the IVT sequences you used in the paper.
I was wondering where you got the modified fast5 m6A from Jenjaroenpun, P. et al. because I did not find a m6A fast5 file in this link :
https://trace.ncbi.nlm.nih.gov/Traces/index.html?view=study&acc=SRP166020
Rania
from cheui.
Hi Rania,
Thanks for testing CHEUI. Yes, if the fast5 are not available to download from the link you will have to contact the authors.
Thanks,
Akanksha
from cheui.
Thank you for your reply.
I already did but they're not reactive :/
Rania
from cheui.
thank you @Akanksha2511
I have one last question, I saw that the reads you used for training models 1 and 2 from Liu et al were obtained with the RNA001 kit. Wouldn't this be problematic since now all the dRNA sequences (as well as the data you used for your tests) are obtained with the RNA002 kit ?
Rania
from cheui.
from cheui.
Hi @EduEyras
thank you for your answer. I was curious because I saw that for Nanopolish polyA they had indeed detected a difference between the two kits [https://github.com/jts/nanopolish/issues/545], but maybe this difference only concerns the polyA tail.
Rania
from cheui.
Related Issues (20)
- transpose expects a vector of size 3. But input(1) is a vector of size 4
- about reference transcriptome file and explanation of cheui output HOT 2
- multi-threading in running model 1
- Regarding output of the m6A probability and stoichiometry command HOT 3
- CHEUI model 2: prediction of stoichiometry and modification probability at transcriptomic sites : Executed with Error HOT 5
- Column header _position_ in site_level_prediction HOT 6
- Regarding CHEUI tool HOT 6
- Issue with the signal rejoining script and model 2 with the 4 dimensional issues HOT 3
- CHEUI preprocess C++ compiling issue
- Low m5C modification site number overlapped with m5c-atlas database HOT 1
- SQK-RNA004 HOT 2
- Regarding prediction of stoichiometry
- Do CHEUI models work on Bacterial RNA modification detection? HOT 1
- Understanding of the read-level prediction file output HOT 1
- pickle data was truncated HOT 4
- Do CHEUI models work on SARS-CoV-2 RNA modification detection? HOT 1
- 2 Questions about model 2 and python/c++ preprocess HOT 1
- Compatibility with the new direct RNA seq kit HOT 2
- CHEUI_preprocess_m5C.py give error HOT 15
- Problem with preprocessm6A HOT 1
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