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pre-mRNA avatar pre-mRNA commented on May 26, 2024 1

Numpy recommendation downgraded to 1.19.5. Thanks Rania!

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EduEyras avatar EduEyras commented on May 26, 2024

Hi,

it should be fine if the TensorFlow is installed properly.

Best

E.

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rania-o avatar rania-o commented on May 26, 2024

Hi @EduEyras

Thanks for your fast reply.
I have another question, I would like to test CHEUI and other tools on the IVT sequences you used in the paper.
I was wondering where you got the modified fast5 m6A from Jenjaroenpun, P. et al. because I did not find a m6A fast5 file in this link :
https://trace.ncbi.nlm.nih.gov/Traces/index.html?view=study&acc=SRP166020

Rania

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Akanksha2511 avatar Akanksha2511 commented on May 26, 2024

Hi Rania,

Thanks for testing CHEUI. Yes, if the fast5 are not available to download from the link you will have to contact the authors.

Thanks,
Akanksha

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rania-o avatar rania-o commented on May 26, 2024

Hi @Akanksha2511

Thank you for your reply.
I already did but they're not reactive :/

Rania

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rania-o avatar rania-o commented on May 26, 2024

thank you @Akanksha2511

I have one last question, I saw that the reads you used for training models 1 and 2 from Liu et al were obtained with the RNA001 kit. Wouldn't this be problematic since now all the dRNA sequences (as well as the data you used for your tests) are obtained with the RNA002 kit ?

Rania

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EduEyras avatar EduEyras commented on May 26, 2024

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rania-o avatar rania-o commented on May 26, 2024

Hi @EduEyras

thank you for your answer. I was curious because I saw that for Nanopolish polyA they had indeed detected a difference between the two kits [https://github.com/jts/nanopolish/issues/545], but maybe this difference only concerns the polyA tail.

Rania

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