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Extract unmapped read fragments from a sequence alignment map by evaluating it's concise idiosyncratic gapped alignment reports (CIGARs)

License: GNU General Public License v3.0

TypeScript 99.96% Shell 0.04%
ngs ngs-analysis ngstools samtools cigar dna bioinformatics bioinformatics-analysis bioinformatics-tool

unmappedcigarfragments's Introduction

unMappedCIGARFragments

Usage

/// <reference path="./lib/lib" />
import {getReads,SAMRead,ReadFragment} from "./lib/lib";

let start = 0;
let end = 10000;
getReads(
    "someSample.sam",
    start,
    end,
    (read : SAMRead,fragments : Array<ReadFragment> | undefined) => {
        //Will be called once for each read who's aligned position begins between start and end inclusive, provided an unmapped fragment is identified.
        //fragments will hold all mapped and unmapped fragments in read (from left to right) identified by evaluating read's CIGAR string against it's query sequence
    }
).then((total : number) => {
    //total will be number of reads with at least one unmapped fragment from 0-10,000 and total number of times callback was called
}).catch(() => {
    //Failed. Likely I/O error
});

unmappedcigarfragments's People

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