This repository contains the Python application and custom czfacsautomation package that have been designed for automating the cell sorting on the Sony SH800S, by the CZ Biohub - SF Bioengineering team. All utilities support Python 3.7. Note that newer versions of Python are not compatible.

This is a public snapshot of the FACS Automation repo as described in our 2023 preprint here.

Maintenance of this repo is the responsibility of Diane Wiener. Please direct any communication to Diane Wiener via creation of an Issue at the project repo here.

This source describes Open Hardware, which is licensed under the CERN-OHL-W v2.

Electronics hardware is described in the facs_electronics folder.

CAD designs are provided in Onshape here.

Software is licensed under BSD 3-Clause.

Copyright Chan Zuckerberg Biohub - San Francisco 2023.

czfacsautomation/hardware/

• HardwareController - Interfaces with hardware peripherals
• ZaberController - Communicates with the zaber stages and gripper
• ArduinoController - Communicates with the arduino via serial

czfacsautomation/sorting/

• Controller - Creates wrapper classes for auto click methods used for interfacing with the Sony SH800S GUI
• Sort - Runs a single sort on the Sony SH800S GUI
• Gating - Runs the GateVertexTool to get and set gate on the Sony SH800S GUI
• CreateGate - Creates a custom gate using the cell population data

czfacsautomation/integration/

• FACSAutomation - Main class to call, starts process, and implements pause/stop

czfacsautomation/slack/

• SlackFacs - Communicates with Slack App to notify users of current run status.

• config/hardware_config.json - Holds the hardware configuration parameters for the zaber stages and arduino
• config/gui_config.json - Holds the config file of all screenshots and parameters needed to run sorting
• config/Slack_config.json - Holds the configuration parameters for the various messages to post

The GateVertexTool vendor/GateVertexTool.exe is available from Sony Biotechnology. Requests to access the GateVertexTool may be made to SONY Biotechnology here. Store the file in the vendor/GateVertexTool/ folder.

1. On Windows OS: Add GateVertexTool folder to "Path" environment variable (read release notes for more details)
2. Create and/or activate a virtual environment in a convenient location with Python3
3. Install and add pytesseract to path
4. Download / clone this repository
5. Navigate to the base of the repository
6. Install setuptools pip install setuptools
7. Install module pip install -e .
8. Create the folder for the exported gating data in the location specified in the "GATE_CSV_DIR" path in the gui_config.json

1. Pull changes from remote repository
2. Activate virtual environment with previous install
3. Navigate to the module directory
4. Update module pip install -e . --upgrade

1. cd to the src directory
2. python -m czfacsautomation "<hardware_config location>" "-facs" ""

1. Chill Tube Housing:

   python -m czfacsautomation "<hardware_config location>" "-chill" ""

2. Run FACS Automation:

   python -m czfacsautomation "<hardware_config location>" "-facs" ""

3. Data Scraper:

   python -m czfacsautomation "<hardware_config location>" "-scrape" ""

4. Hardware Demo:

   python -m czfacsautomation "<hardware_config location>" "-demo" ""

5. Calibration:

   a. Calibration Update Scan:

   python -m czfacsautomation "<hardware_config location>" "-calibrate" "-s"

   b. Tube Calibration Check:

   python -m czfacsautomation "<hardware_config location>" "-calibrate" "-t"

   c. Full Calibration Routine (Scanning and with Tubes):

   python -m czfacsautomation "<hardware_config location>" "-calibrate" "-f"