Chris Ulpinnis & Pascal Püllmann 2018-10-11

The Golden Gate cloning technique has been proven to be a highly efficient toolbox for a variety of cloning setups. Based on its modular concept it is particularly suitable for the use in multiple-site mutagenesis approaches. In this technical note we developed a protocol termed Golden Mutagenesis for the rapid, easy, reliable and cheap formation of mutagenesis libraries. One to five positions could be altered in parallel or simultaneously within two days. To facilitate the implementation of this technique, this R-library has been developed for the automated primer design and the graphical evaluation of sequencing results to determine the quality of the library.
This library is currently still under development and will be enhanced by an R shiny web-application.

You can install GoldenMutagenesis from github with:

# install.packages("devtools")
devtools::install_github("ipb-halle/GoldenMutagenesis")

To start with this library we recommend to have a look on our vignettes!
Point Mutagenesis
Multiple Site Directed Mutagenesis - Example1
Multiple Site Directed Mutagenesis - Example2
Multiple Site Directed Mutagenesis - Example3
Quick Quality Control

You can try our webtool at http://msbi.ipb-halle.de/GoldenMutagenesisWeb or on binderhub at .

The Webtools git repository is available at: https://github.com/ipb-halle/GoldenMutagenesisWeb

Please note that the webtool is still in beta phase. There are still errors and also the user interface is not the final user experience. If you encounter any problems please send an email to me.