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sfchen avatar sfchen commented on May 19, 2024

Your zlip is too old that it doesn't have functions like gzbuffer or gzoffset.

You can update the zlip, or just download the binary distribution from http://opengene.org/fastp/fastp , and run chmod a+x fastp to make it executable.

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wdecoster avatar wdecoster commented on May 19, 2024

Alright, thanks for the quick reply.

Running fastp on a fastq.gz file of nanopore data throws an error, not sure why:

$ fastp -t 20 -i fail/fastq_runid_fff19c6dcf86112967c3df0777ebeddda1109a40_1.fastq.gz -q 8 -l 5000 -o out
Detecting adapter for single end input...
No adapter detected

terminate called after throwing an instance of 'std::system_error'
  what():  Enable multithreading to use std::thread: Operation not permitted
Aborted (core dumped)

Perhaps would be more appropriate in a separate thread, but do you think it would be possible to have fastp read from stdin and write to stdout?

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sfchen avatar sfchen commented on May 19, 2024

Thanks for your information.

I compiled that binary on Ubuntu, and seems it is not working on centOS.

I recompiled one in CentOS, you can download it from: http://opengene.org/fastp/fastpcentos

And another option is to update your old zlib, then compile by your self.

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sfchen avatar sfchen commented on May 19, 2024

And I also haven't tried any nanopore data, could you send me one or upload one here? (You can upload only first 1000 lines)

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wdecoster avatar wdecoster commented on May 19, 2024

I'll test fastp again. I attached 1000 reads of E.coli data, but you can find plenty more publicly available, e.g.
https://github.com/nanopore-wgs-consortium/NA12878
nanopore.fastq.gz

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wdecoster avatar wdecoster commented on May 19, 2024

fastp works fine now!

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