Comments (6)
Your zlip
is too old that it doesn't have functions like gzbuffer
or gzoffset
.
You can update the zlip
, or just download the binary distribution from http://opengene.org/fastp/fastp , and run chmod a+x fastp
to make it executable.
from fastp.
Alright, thanks for the quick reply.
Running fastp on a fastq.gz file of nanopore data throws an error, not sure why:
$ fastp -t 20 -i fail/fastq_runid_fff19c6dcf86112967c3df0777ebeddda1109a40_1.fastq.gz -q 8 -l 5000 -o out
Detecting adapter for single end input...
No adapter detected
terminate called after throwing an instance of 'std::system_error'
what(): Enable multithreading to use std::thread: Operation not permitted
Aborted (core dumped)
Perhaps would be more appropriate in a separate thread, but do you think it would be possible to have fastp read from stdin and write to stdout?
from fastp.
Thanks for your information.
I compiled that binary on Ubuntu, and seems it is not working on centOS.
I recompiled one in CentOS, you can download it from: http://opengene.org/fastp/fastpcentos
And another option is to update your old zlib, then compile by your self.
from fastp.
And I also haven't tried any nanopore data, could you send me one or upload one here? (You can upload only first 1000 lines)
from fastp.
I'll test fastp again. I attached 1000 reads of E.coli data, but you can find plenty more publicly available, e.g.
https://github.com/nanopore-wgs-consortium/NA12878
nanopore.fastq.gz
from fastp.
fastp works fine now!
from fastp.
Related Issues (20)
- I obtained different 'insert size peaks' when I used different options for the same paired-end sequencing sample.
- Insert size estimation and report interpretation HOT 2
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- Feature request: add option to set lower limit of unqualified quality
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- Interpretation help file?
- Store duplicate reads
- Split interleaved output
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- Keep occurred error message from the beginning < igzip: invalid gzip header found >
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- No adapter detected for read and Q20 bases: 4747174600(99.9999%)
- fastp not removing all Illumina universal adapter sequences as indicated by FastQC HOT 2
- few options throw 'undefined error' -reg
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from fastp.