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ThorstenFalk avatar ThorstenFalk commented on September 20, 2024

Hi Jim,

U-Net can in principle be used for natural images like PASCAL VOC and the like but there are much better models around. I would definitely first check the literature for alternatives with existing comparisons to (vanilla) U-Net.

If you have MATLAB you can use the MATLAB scripts in U-Net: Convolutional Networks for Biomedical Image Segmentation to generate the blobs including augmentation.

However, most of the augmentations are not very useful for natural images, e.g. compared to microscopic images image orientation is an important cue for natural images. Also local image deformations are most often harmful. You also encounter scale variations due to the perspective camera, self-occlusion and object cropping at the image boundaries. A mirroring across the boundaries as was done or the EM recordings does not work for natural images.

So if you want to use U-Net, pad out-of-image regions with a special value e.g. zero, turn off elastic deformation and rotation. Instead add RGB augmentations, like white-point shifts and Poisson/Gaussian noise augmentation. The receptive field of the vanilla U-Net with four resolution levels is approximately 184x184 pixels, objects exceeding this size especially if they do not show some characteristic texture will not be properly segmented. So you have to play with the number of resolution levels and with more classes more feature channels in the decoder may be required.

All the best,

Thorsten

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JimHeo avatar JimHeo commented on September 20, 2024

Hi Thorsten,

I had the vacation. Sorry for the late reply.
I understand that natural images are not proper to use U-Net.

Thanks.
Regards,
Jim

from unet-segmentation.

ThorstenFalk avatar ThorstenFalk commented on September 20, 2024

weights are for class weighting and extra-weights on instance boundaries for instance segmentation, weights2 are the pdf from which the center point of input patches is sampled. Please read the supplements of our Nature methods paper for details.

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JimHeo avatar JimHeo commented on September 20, 2024

I missed that supplements,,,
Appreciate it!

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