Comments (1)
Hello, you appear to have done something I have never tried. And I think we'll have to call it a bug. You do not appear to be using a sequence (FASTA file). This means my code doesn't have a reliable way to determine the length of your chromosome. My code tries to make a guess by selecting the greatest value from the VCF file. You have an annotation beyond the greatest position in your VCF and this throws the error you've reported. At this point I think we have several options.
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You could use your reference. You used this to to call variants, so you must have something. This will allow the code to git the chromosome length from this sequence.
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You can omit the offending annotations. Something like:
vcf <- read.vcfR('example.vcf')
len <- max(as.numeric(as.character(vcf@fix[,'POS'])))
gff <- read.table("example.gff", sep='\t', quote='')
gff2 <- gff[gff[,4] <= len,]
gff2 <- gff2[gff2[,5] <= len,]
This would remove some of your annotations, so its suboptimal. But it works.
- You could try the devel branch from GitHub, if you're into that sort of thing. I've used your example to engineer my function so that it can handle this sort of thing. It will eventually end up on CRAN. But as of today is only at GitHub. Thank you for helping me improve vcfR!
I hope that helps!
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Related Issues (20)
- Variants with MAF of 0 after removing monomorphics HOT 6
- Error: ID column contains non-unique names HOT 1
- How can I add population information to genind converted using vcfR2genind? HOT 2
- RStudio Session aborted when writing VCF
- vcfR not recognizing all chromosomes HOT 2
- Heterozygous SNP calling HOT 15
- read.vcfR() coredump with empty lines in input HOT 5
- Rename Genotype information HOT 2
- vcfR2tidy can't handle files with empty INFO HOT 4
- gene/chromosome annotation HOT 2
- write.vcf() not filtering variants HOT 2
- Filtering for minor allele Frequency HOT 4
- Assign_Indiv_by_Geno.R function of Demuxafy.sif problems with the vcf HOT 1
- chromR object not finding DP and MQ values HOT 2
- read.vcfR() not recognizing missing data HOT 3
- extract.gt() missing heterozygotes HOT 1
- compilation issue HOT 2
- Hide a panel from chromoqc or plot HOT 2
- vcfR Subsetting to the first chromosome HOT 1
- change genotype(change gap to NA) HOT 2
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